FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10678991
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG158990.1488811068386517TruSeq Adapter, Index 16 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC36650.052.03577833
CGTATGC38300.047.8507144
TATGCCG39750.046.54393446
GTATGCC43200.043.1434545
GCACACG48350.041.51313411
ATGCCGT45750.040.21666747
TCGTATG45500.040.1257143
ACGTCTG50750.039.6173515
CCGTCCA48300.039.34282334
CACACGT51950.038.8357212
CACCCGT51000.037.52887731
ACACGTC54500.037.0186413
TCACCCG52250.036.3015630
GTCACCC52800.036.24999229
ACCCGTC53100.036.04451432
CACGTCT58250.034.99053614
CTCGTAT48800.034.227242
TGCCGTC53250.034.16435648
CGTCCAT56150.031.81596635
AGCACAC64650.031.7400410
Produced by FastQC (version 0.11.2)