FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9998326
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA172850.17287893993454506TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38850.048.60188748
CGTATGC39150.048.31351546
GTATGCC42150.045.20442247
GCACACG55450.043.7865911
ACGTCTG56900.042.6118615
ATGCCGT44250.041.57993349
TCGTATG45450.041.30992545
ACACGTC59850.040.68358613
CACACGT59900.040.6496312
CACGTCT62950.038.57149514
CTCGTAT52050.036.93231244
AGCACAC68500.035.69650310
GTCACAT67650.035.0737329
GCCGTCT49200.034.87265851
TGCCGTC52900.033.80313550
ATGTCAG71350.033.20740534
CGTCTGA74350.032.84276216
CACATGT72100.032.76531
TCCAGTC74350.032.74851225
TCTCGTA61550.031.95864743
Produced by FastQC (version 0.11.2)