FastQCFastQC Report
Wed 20 Dec 2017
HF7T3BGX5_n02_musca_male_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7T3BGX5_n02_musca_male_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31434997
Sequences flagged as poor quality0
Sequence length50
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT597350.19002705805888895No Hit
CAGTAGTAGTATTCTAAGAATATACATATTTCTTTCTCACATGGAAAGCC545820.17363450042638784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG541740.1723365839672261No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT462570.14715127855746257No Hit
TTATTACACACACAGTAGTAGTATTCTAAGAATATACATATTTCTTTCTC333530.10610148936868039No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT329830.10492445728561704No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT322250.10251313209923323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGGG125300.036.867221
TAGTGAA473550.035.1689641
GCGGACC21600.031.36637344
TTGAATG372600.029.3358271
TAGATCG99950.028.89676944
CCGCGGC52800.028.16307844
CCGTACT94750.027.371622
GCGGAGC41800.027.36493344
TAGATCT421800.026.41436844
TAGCTCG55900.026.36515644
TCTCGGG37600.025.50738544
GTAGATC555500.025.19234843
GTATCAA260800.024.59531
GCGTGTC69750.024.37825644
CCCGTAC40400.024.2843041
TGTAGCG48900.023.7965644
GCCGTAC42050.021.7620331
TTATCAA292250.020.5033471
GTAGCTC162300.019.84182443
TAAGTCC92900.019.67634243
Produced by FastQC (version 0.11.2)