FastQCFastQC Report
Wed 20 Dec 2017
HF7T3BGX5_n02_musca_female_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7T3BGX5_n02_musca_female_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29631850
Sequences flagged as poor quality0
Sequence length50
%GC37

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG685830.23145028069459045No Hit
GACGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCT343560.11594281153556056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGGG78300.030.14511
TAGTGAA229900.026.5714741
GAGTACG109850.023.7700121
TAGATCT281450.023.56382244
GCGGACC14750.022.81702844
GTAGATC358950.022.64290243
CCGCGGC30800.021.56829844
CCGTACT64200.021.4832632
TAGATCG71400.021.28822744
TCTCGGG28900.020.85510644
TAGCTCG35750.020.79698444
AGTACGT153200.020.5900842
GTATCAA163850.020.2054621
TTGAATG264550.019.7485221
TTATAGG33800.018.5484181
GCGTGTC47350.017.32800344
CCCGTAC36200.017.1971551
CGGTTCA61900.017.05968934
TCAACGC429050.016.9917743
GCGGAGC28800.016.95587344
Produced by FastQC (version 0.11.2)