FastQCFastQC Report
Wed 20 Dec 2017
HF7T3BGX5_n01_musca_male_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7T3BGX5_n01_musca_male_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31434997
Sequences flagged as poor quality0
Sequence length20
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG8447352.687243774828418No Hit
GTCCTGTCTCTTATACACAT1259910.40079851128982136No Hit
GTGCTGTCTCTTATACACAT951600.30271992709272405No Hit
GTACTGTCTCTTATACACAT581000.1848258487188658No Hit
GTTCTGTCTCTTATACACAT560820.17840625211448247No Hit
CTGTCTCTTATACACATCTC414690.13191984716906446No Hit
GTACCTGTCTCTTATACACA370050.1177191141452948No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC38850.011.8604741
GTACTCC45850.011.8519421
GTACTCA46050.011.6940191
GTCCTAC49200.011.5146451
GTACTGC63400.011.420851
GTGCCCC78850.011.4032971
GTACTGT105400.011.3611581
GTCCCAA78950.011.3090251
GTGCACC55750.011.2420441
GTACTAT40850.011.1597911
GTACTTA34700.011.0590591
GTCTCAC51950.011.0533891
GTGCAAC50500.010.9824921
GTCTCCA59800.010.9607891
GTCTATA35250.010.9461041
GTACCTA48900.010.9265441
GTACGTA48200.010.9254151
GTCCGCA47550.010.8980381
GTACCCC69100.010.8942561
GTACTCT35400.010.8799421
Produced by FastQC (version 0.11.2)