FastQCFastQC Report
Wed 20 Dec 2017
HF7T3BGX5_n01_musca_female_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7T3BGX5_n01_musca_female_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29631850
Sequences flagged as poor quality0
Sequence length20
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG8186772.7628278355890705No Hit
GTCCTGTCTCTTATACACAT710140.23965429090657517No Hit
GTGCTGTCTCTTATACACAT522410.17630016350649724No Hit
GTAAGCAGTGGTATCAACGC497880.16802190885820495No Hit
GTACTCCTGTCTCTTATACA448670.15141477835504702No Hit
GTACTGTCTCTTATACACAT415670.1402781129089139No Hit
GTACCTGTCTCTTATACACA326200.11008425056147356No Hit
GTTCTGTCTCTTATACACAT319860.10794466089697402No Hit
CTGTCTCTTATACACATCTC310990.10495126021493764No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC333650.013.6724613
GGGCCAA575100.013.6685624
GTATACC298350.013.66754151
ATATCAA234700.013.641736
TATACCA295150.013.6166412
CGGGCCA561100.013.5668523
GTTTTCA220200.013.56030751
TTTTCAA223900.013.5506492
GCCAAGT579850.013.5433126
CAAGTGA577100.013.4603818
TACCACA341100.013.388094
ACCACAC340050.013.3820865
TATATCA143600.013.3610635
TTCAATA246650.013.3497334
AAAAACT96550.013.3029358
CGTATAT141550.013.2975313
AACTTCA160650.013.284337
CCAAGTG591900.013.2645147
TCGGGCC496050.013.2486892
ATAAGTC247100.013.2142528
Produced by FastQC (version 0.11.2)