FastQCFastQC Report
Thu 21 Dec 2017
HF7T2BGX5_n02_musca_male_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7T2BGX5_n02_musca_male_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36763525
Sequences flagged as poor quality0
Sequence length50
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30233988.223906711883586No Hit
CAGTAGTAGTATTCTAAGAATATACATATTTCTTTCTCACATGGAAAGCC685750.18652999134332193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAA625150.037.157841
TAATGGG163650.036.8128781
TAGCTCG157050.034.6754244
TTGAATG470850.031.449971
GTAGCTC280150.027.45750843
TGTAGCG128200.027.37518744
TTATCAA349050.024.7302151
TGTAGCT369550.021.0946542
GGTATCA77150.021.0607451
GCCGTAC69050.020.8884091
TGTATCA104650.020.2326281
GCGGACC27000.019.96580544
CCCGTAC65900.019.784931
TAGCGCG32300.019.61888944
CCGTACT181300.019.599042
GTATCAA260350.019.5674251
GTAGATC132800.018.60638443
TATCAAC778300.018.041522
GGAGCTC86500.017.78039443
GTAGCGC39950.017.5692943
Produced by FastQC (version 0.11.2)