FastQCFastQC Report
Thu 21 Dec 2017
HF7T2BGX5_n02_musca_female_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7T2BGX5_n02_musca_female_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34230742
Sequences flagged as poor quality0
Sequence length50
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22130996.465238176841156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGGG99200.030.521261
TAGTGAA280300.030.1223131
TAGCTCG103500.030.1024744
TGTAGCG85500.023.6241344
GAGTACG88000.022.687331
TTGAATG308400.022.5224421
GTAGCTC217250.020.91396143
TTATAGG42650.017.7860811
AGTACGT128250.017.5222632
TCAACGC431950.017.386713
GCGGACC16800.017.28796644
CCGTACT118500.017.164062
GTATCAA159500.016.887121
CGGTTCA85650.016.61925934
GCCGTAC51700.016.458891
TAGCGCG20900.016.31791544
ATCAACG463500.016.1154522
CCCGTAC45500.016.092111
TGTAGCT287650.016.00947642
TTATCAA240700.015.9678161
Produced by FastQC (version 0.11.2)