FastQCFastQC Report
Thu 21 Dec 2017
HF7T2BGX5_n01_musca_male_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7T2BGX5_n01_musca_male_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36763525
Sequences flagged as poor quality0
Sequence length20
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG29891628.130781800711437No Hit
GCGGGGGGGGGGGGGGGGGG9247072.515283830916649No Hit
TGGGGGGGGGGGGGGGGGGG1587460.43180298951202317No Hit
CGGGGGGGGGGGGGGGGGGG1557000.4235176033854207No Hit
CCGGGGGGGGGGGGGGGGGG1186730.3228009283658191No Hit
TCGGGGGGGGGGGGGGGGGG760060.20674296058389396No Hit
ACGGGGGGGGGGGGGGGGGG628050.1708350872230016No Hit
GTCCTGTCTCTTATACACAT574050.1561466154292876No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT401400.011.732203514
GCGGGGG1169250.011.1345341
CCCAAAA46900.010.3287833
GTACTCC25500.010.2401241
AAAATTA35250.010.1877366
GTCCCAA55400.09.9954711
GTACTAC21350.09.93530751
GTACTCA29350.09.8032551
TACAAAA30850.09.8023522
CCAAAAT39450.09.7950934
ATACACA481950.09.77870213
GTACTGC39250.09.7384441
AAATTAC38950.09.668777
GTCTCAC34250.09.6680111
CGGGGGG1733900.09.5701252
ACAAAAG33300.09.5229143
GTCGTAC21850.09.5157181
GTGCCCC53650.09.4211531
GTCTCCA37600.09.4210481
GTCCGCA34500.09.4153271
Produced by FastQC (version 0.11.2)