FastQCFastQC Report
Thu 21 Dec 2017
HF7T2BGX5_n01_musca_female_dropseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7T2BGX5_n01_musca_female_dropseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34230742
Sequences flagged as poor quality0
Sequence length20
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG24498737.15693805293499No Hit
GCGGGGGGGGGGGGGGGGGG7111672.0775681695710833No Hit
TGGGGGGGGGGGGGGGGGGG1289000.37656209731007295No Hit
CGGGGGGGGGGGGGGGGGGG933370.27267010455104945No Hit
CCGGGGGGGGGGGGGGGGGG735100.21474848544036818No Hit
TCGGGGGGGGGGGGGGGGGG606150.1770776689561681No Hit
ACGGGGGGGGGGGGGGGGGG476840.13930168384897998No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCAA621350.013.7010044
ATACCAC359300.013.6537463
ATATCAA253050.013.616246
CGGGCCA619250.013.5935863
GCCAAGT627700.013.5847846
TATACCA270950.013.5609372
CAAGTGA626950.013.488518
ACCACAC361700.013.4549455
TACCACA364950.013.4309924
GAAGAAT160400.013.3766325
CCAAGTG641400.013.3243047
TTTTCAA204450.013.2913992
CCACACG370850.013.2891226
GTATACC170100.013.2727021
CGTATAT150900.013.272223
ATAAGTC268500.013.2620748
GAACTTC178500.013.2438666
TCGGGCC503000.013.2430712
CCTGAAC177450.013.23522953
TGAAGAA160750.013.2342424
Produced by FastQC (version 0.11.2)