FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32008586
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG713891722.30313141605193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG38600.056.94886821
GCTATCG38650.056.5137320
TAGCTTA75300.055.8511121
TACCCCG37800.054.16318533
GTATAGT76500.053.5253915
GGTAGTA111750.053.4726834
CGTTCGC25050.053.37549625
TGTATAG80000.051.9272614
TTGTATA79850.051.76135613
CCGTAGT26050.051.733585
TATCGGG42900.050.83289722
GACTGAT84150.050.69692211
GAGGTAG129050.050.0243532
GTTCGCC25900.049.86205326
AGGTAGT118400.049.850733
TAGTTAA70050.049.4649718
CGTAGTG27550.048.915116
TGAGGTA129250.048.712781
GTTGTAT65950.047.6529912
CCGAACC40400.047.480820
Produced by FastQC (version 0.11.5)