FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30121082
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG558680.1854780648318012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG17450.013.6388153
CGTATTA11450.013.4504763
TACGTAC20950.011.36023252
GACGTAT22400.010.46854217
CCCGTAT38800.010.4657761
CGTTACG19100.010.44494148
ACGTATA22850.010.415894518
CTCGTAT60250.010.2258841
TCGACCG19950.09.99978527
CTCGCGT10550.09.6225631
TACGAAA24700.09.49399156
CTACGTA26550.09.49141651
CGTATAG24950.09.39951619
CGTATCG25000.09.37981417
CTCCGAT76500.09.2434731
GTCGCGA7201.7498678E-99.2377531
CCCGTCT79100.09.2051751
GTACACT107250.09.2044331
CGGCATT43100.09.1779661
CTCCGCT104300.09.028451
Produced by FastQC (version 0.11.5)