FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29349121
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG648110.2208277379073806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG18400.011.98346253
CGTATTA12150.011.8113953
TACGTAC21750.010.78143552
CCCGTAT32200.010.2192631
GTCCGGT35900.09.84861
CGGGAAT41950.09.5130651
TACGAAA25900.09.05406356
CTCGTAT53700.08.9308731
GATCGCA27650.08.86063227
CTCCGCT99700.08.8130491
CCCGTCT78050.08.7011451
CGGCATT40850.08.6551961
CTCCGAT75200.08.611951
CGTTACG15850.08.6118248
CGCTAGT10203.6379788E-128.5800041
CGTATCG25300.08.5770117
CTACGTA28600.08.56626651
TGCTACG29600.08.51337649
CGTTTCG17400.08.4480431
GACGTAT22000.08.43175417
Produced by FastQC (version 0.11.5)