FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29730629
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG650720.21887192497676383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG19100.012.64403553
CGTATTA13000.012.1158663
CCCGTAT35300.011.8011421
CGTATCG26150.011.64403917
CGTTACG19250.011.27271948
TACGTAC22550.011.01996752
GTCCGGT33900.010.9460641
CGTTTCG17200.09.97057231
TACGAAA25700.09.94169556
CTACGTA28650.09.52877351
GACGTAT22100.09.34363417
CTCGTAT55650.09.184161
GATCGCA29000.09.0514927
TCGTTAC32550.09.03226847
TGCTACG30100.08.95351349
CCCGTCT75250.08.9319541
GTACACT108750.08.8522821
TCGTATC45750.08.4922272
GTCCGTA25850.08.3962021
GTCGGTT61050.08.3718021
Produced by FastQC (version 0.11.5)