FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32794680
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG717370.21874584536272346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26200.020.70592948
CGTATTA12950.013.2438373
TCGTATG45300.011.8981945
CCCGTAT38250.010.9823071
CGTATGC50000.010.49979846
ACGTACG19400.010.28337153
CGGGAAT43850.09.180621
GTCCGGT35350.09.1104991
GACGTAT24250.09.09276217
CTCCGCT114950.08.861921
TACGTAC23700.08.41770152
GTCCGTT49450.08.3533281
CTCGTAT62650.08.21215744
GTCGCAT26200.08.1502761
ATGCCGT72900.08.11377349
CGGCATT49150.08.0481991
ACGTATA27450.08.03298818
CTCCGAT81650.08.0173211
CCGGCAT59850.08.0130941
TACGAAA27650.07.97476156
Produced by FastQC (version 0.11.5)