FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31398368
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG611690.19481585794522824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18800.016.19663248
CGCTACT18000.010.5016091
CCCGTAT39450.010.2043511
ACGTACG18200.010.19206753
CGTTACG20050.09.60089448
CCCGTCT80300.09.459731
CGTAGTA54550.09.43155266
GACGTAT23800.09.41176817
AATCCGA62400.09.25542924
CGTATTA12050.09.004743
GTCCGGT33950.08.8673391
CTCGTAT63500.08.8202491
CCCGTTT69850.08.8202491
TACGTAC20400.08.74984952
ACGTATA26700.08.65185418
CGGCATT40950.08.5483181
TCGTATG39050.08.51456445
CTCGAAT110800.08.3722211
CGCTAGT10901.8189894E-128.3499031
CGCGTAA13500.08.29621970
Produced by FastQC (version 0.11.5)