FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29084535
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG665370.2287710633847163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT186500.018.5443761
TATGCCG18950.016.807248
TGTAGCT292500.014.7055157
TACACTG231300.014.2549642
CGTTACG63000.013.55540548
TAGCTGT312250.013.3047819
ACTGTAG280750.013.2899885
ACACTGT257700.013.1341323
CACTGTA293700.012.7278314
TCGTTAC80650.012.67189947
CTGTAGC288650.012.6592896
GTAGCTG343700.012.036348
CGTATCG29200.011.62664317
AGCTGTC358350.011.55411810
ACGTACG21250.011.36452153
CTCGTTA101500.011.31029646
GTTACGG128150.011.17039849
ACCTTCG89550.010.86544861
TTACGGA162000.010.84559550
TTCAGAC332650.010.52150617
Produced by FastQC (version 0.11.5)