FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26620734
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG456190.1713664243818371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28200.034.6271946
TCGTATG45500.021.76905843
CGTATGC49400.019.62538744
ACGTACG19550.015.39629753
CTCGTAT60400.014.54458642
ATGCCGT70400.014.31804247
GTATGCC74100.013.50856445
TACGTAC23750.013.11568552
GCCGTCT77750.012.73942549
TGCCGTC82900.012.32793848
TACGAAA26650.012.08250156
GTCGCAT20950.011.3624261
CGTATCG27300.010.89736917
CTACGTA29050.010.84324751
AATCTCG87000.010.25836639
GATCGCA31500.010.11089827
TGCTACG30600.010.0652649
CGTACGA30700.09.91858154
GTAATCT88900.09.76348237
TCTCGTA88000.09.74416741
Produced by FastQC (version 0.11.5)