FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25624568
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG545230.21277627002336194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24400.028.68820446
TCGTATG40250.017.04312143
CGTATGC48950.014.37159144
CGTTACG18350.011.82550348
CTCGTAT54500.011.68796442
ATGCCGT62650.011.56407147
CGTATTA9850.011.0159873
GTACACT94900.010.9186561
GTATGCC68200.010.82823945
TGCCGTC71050.010.1969548
ATTCGCG10650.010.1881956
CCCGTAT30900.09.9693741
CGGCATT35350.09.9027151
GCCGTCT74550.09.76516849
GTCCGGT28900.09.2057561
CGGGAAT35750.09.20441
CGTAGTA41200.09.08970466
ACGTACG13100.08.81667853
GTCGCAT19200.08.7515251
GTTACGG46850.08.740581549
Produced by FastQC (version 0.11.5)