FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24620396
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1192300.48427328301299466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37400.049.31725746
TCGTATG53100.035.79014243
TATCTCG52600.033.73476839
CGTATGC59150.031.83372344
CGCCAAT63500.029.54233433
ACGCCAA63450.029.2900532
GTCACGC65100.028.38673829
CTCGTAT72550.024.98924642
ATGCCGT75050.024.71654347
AGTCACG75900.024.53196728
GTATGCC78950.023.67276845
CCAATAT80500.023.39053335
GCCGTCT82200.022.73689349
TCACGCC87800.021.60560630
TGCCGTC87600.021.53511648
GCCAATA89200.021.22696334
ATATCTC87650.020.32465438
TCTCGTA94500.019.33289141
CGTCTGA101000.019.02443516
ACGTCTG100300.019.01770415
Produced by FastQC (version 0.11.5)