FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb202.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb202.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28502573
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG518890.18205023104405346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24350.031.19054246
TCGTATG43100.018.18991343
CGTATGC47150.016.70167244
CGTTACG15750.012.88869248
ATGCCGT68000.011.78658447
GCCGTCT78100.010.39673149
GTATGCC74900.010.373645
CTCGTAT64500.010.36420242
GTCCGGT33000.010.2897421
GTCGCAT19400.010.285371
TGCCGTC83750.010.07148748
GTACACT109050.010.015571
CCCGTAT31300.09.3946731
CCCGTCT73650.09.2209511
CGTAGTA45850.09.08394266
GTCGAGT26350.08.9010441
GTCCGTT47850.08.7058611
GTTACGG41200.08.66488649
GTCCGCA53450.08.4486781
CGGGAAT40050.08.3036271
Produced by FastQC (version 0.11.5)