FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27001931
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG501170.18560524430641645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25700.033.50142346
TCGTATG46650.020.2569243
CGTATGC53200.016.84196744
CTCGTAT61550.014.32956342
TTACGCG2405.5729593E-513.12489256
TCGACCG34350.012.83810931
CGTTACG23300.012.76801848
ATGCCGT67950.012.72241547
GTATGCC75900.011.71261945
GCCGTCT76400.011.68173349
ACGTACG22750.011.53821853
TACGTAC24300.010.802352
GTACACT113100.010.6780251
ACGTCTG86750.010.65107215
CGTCTGA88850.010.63567816
TGCCGTC84700.010.57832848
GACCAAT87150.010.52180835
CGTGCGA18850.010.21204338
ACACGTC93050.010.15557813
GTCGCAT19200.010.0275751
Produced by FastQC (version 0.11.5)