FastQCFastQC Report
Fri 10 May 2019
HF7M2BGXB_n01_mb200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7M2BGXB_n01_mb200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31518045
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG650680.20644681483258243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT180450.016.6837161
ACGTACG41850.015.6390853
TACGTAC42850.015.35573852
CGTATCG60450.014.6482317
TATGCCG17650.014.07913546
TACGAAA47700.014.01471156
CTACGTA47500.013.92615351
GATCGCA50700.012.97811227
TGTAGCT294750.012.9426327
CGTTACG53400.012.84627248
TGCTACG51250.012.83883449
TACACTG230400.012.7763712
GCTACGT51850.012.75780750
ACGAAAC54350.012.42873557
TCGACCG83050.012.38978631
ACCCCGG86500.012.34111270
AGATCGC54700.012.285068526
TAGCTGT297150.012.2727689
GTCGCAT19850.012.1685761
CGAAACC53700.012.12301458
Produced by FastQC (version 0.11.5)