FastQCFastQC Report
Sat 25 Feb 2023
HF7LVAFX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7LVAFX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179874652
Sequences flagged as poor quality0
Sequence length10
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCCAC17109458995.11878805469489No Hit
AGATCTCGGT40845052.270750744801997Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG6168800.3429499338239164No Hit
CTCCTGCCCC2042340.11354240173873972No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)