FastQCFastQC Report
Sat 25 Feb 2023
HF7LVAFX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7LVAFX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179874652
Sequences flagged as poor quality0
Sequence length10
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTTCGGGT16841168293.62724548870844No Hit
GGGGGGGGGG68738233.8214517296189126No Hit
TGGTTCGGTA6397410.35565933992745125No Hit
TGTTCGGGTA6029950.3352306694108295No Hit
TGGTCGGGTA3829410.2128932541312158No Hit
GGTTCGGGTA3268690.18172043496156423No Hit
TGGTTGGGTA2155630.1198406766062847No Hit
TGGTTCGGGG2146530.11933476874773884No Hit
TGGTTCGGGA2077080.1154737466844411No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)