FastQCFastQC Report
Fri 20 Sep 2024
HF7LJDRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7LJDRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978652392
Sequences flagged as poor quality0
Sequence length24
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTGGTCGCCGTATCAT143689851072.62005776303127Illumina Single End PCR Primer 1 (100% over 24bp)
TGATCTCGGTGGTCGCCGTATCAT26527275213.40673849901777Illumina Single End PCR Primer 1 (95% over 24bp)
TGAGCTCGGTGGTCGCCGTATCAT42100590.21277405859775697No Hit
AGAGCTCGGTGGTCGCCGTATCAT25732650.13005139307965924Illumina Single End PCR Primer 1 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)