FastQCFastQC Report
Fri 20 Sep 2024
HF7LJDRX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7LJDRX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978652392
Sequences flagged as poor quality0
Sequence length10
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGATAT24356944512.309865339904533No Hit
CTCCTCTAAT20032001610.124063064837717No Hit
GGGGGGGGGG1530230027.733698077474135No Hit
CGGAATATCT635951313.214062826655406RNA PCR Primer, Index 37 (100% over 10bp)
GACGACATCT529891992.6780448761108113RNA PCR Primer, Index 41 (100% over 10bp)
ACTGATATAT517595782.6159005093199816No Hit
ATTCCTTTAT513423862.5948158558615586No Hit
GTGATCCAAT501654642.535334867449522No Hit
CTAGCTATCT500736662.5306954471869663RNA PCR Primer, Index 38 (100% over 10bp)
CATTTTATCT499404362.5239620765080804RNA PCR Primer, Index 35 (100% over 10bp)
TACAGCATCT498653982.5201696973967525RNA PCR Primer, Index 43 (100% over 10bp)
CTATACATCT494772702.500553922459767RNA PCR Primer, Index 39 (100% over 10bp)
GTGGCCTTAT493058182.4918888329931574No Hit
CAAAAGATCT477884852.415203660492176RNA PCR Primer, Index 28 (100% over 10bp)
TAATCGATCT460162002.325633354602894RNA PCR Primer, Index 42 (100% over 10bp)
ATGAGCATCT448330212.2658361408636956RNA PCR Primer, Index 26 (100% over 10bp)
GAGTGGATAT443345032.2406413162438894No Hit
CCAACAATCT425498902.1504479600376416RNA PCR Primer, Index 36 (100% over 10bp)
CAACTAATCT412819522.0863670732115134RNA PCR Primer, Index 29 (100% over 10bp)
TCGAAGATCT403092212.0372057852595264RNA PCR Primer, Index 47 (100% over 10bp)
GTGAAACGAT399774502.0204382620027177No Hit
CACGATATCT396219812.002473054903319RNA PCR Primer, Index 31 (100% over 10bp)
GTTTCGGAAT390099641.9715420534563506No Hit
GGTAGCATCT385127341.9464123236457798RNA PCR Primer, Index 24 (100% over 10bp)
CACCGGATCT380863121.9248611910808031RNA PCR Primer, Index 30 (100% over 10bp)
TCCCGAATCT373144701.8858527223310277RNA PCR Primer, Index 46 (100% over 10bp)
CACTCAATCT364824161.8438011723284038RNA PCR Primer, Index 32 (100% over 10bp)
CGTACGTAAT356711801.802801752557657No Hit
TATAATATCT338411281.7103119343662865RNA PCR Primer, Index 44 (100% over 10bp)
CAGGCGATCT337175401.704065865046598RNA PCR Primer, Index 33 (100% over 10bp)
CATGGCATCT327914061.6572595637607073RNA PCR Primer, Index 34 (100% over 10bp)
TCATTCATCT325052261.6427961844851422RNA PCR Primer, Index 45 (100% over 10bp)
TCGGCAATCT317276301.6034969117506317RNA PCR Primer, Index 48 (100% over 10bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)