FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n02_Neg_Water_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n02_Neg_Water_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCCAGAATCGGAATTTGAGCTGAATTTTCAAAAGCAAGTAGGCTGTG15.263157894736842No Hit
TTTATTTTCTTCACCATATATACACCATCACTTTAATAACACCTTCCAAA15.263157894736842No Hit
TATGATTTCTGCCATCATGTCTGAAGTTATTCCCTCCGTAATTCCAGTGA15.263157894736842No Hit
AGACTGAACCCTTAACCTGTGGGAACCAATGCTGCCTCAGGGTAGCGGGC15.263157894736842No Hit
GCCAGACAACTACCTGCCCTCCACCCCTCTAATATTCAGAAATCAAATGT15.263157894736842No Hit
GCCCGCGGCACGAGGTCCGCTGCCTTCGGCCAGGTGGTCATCTGTGGCGG15.263157894736842No Hit
TATTTAGTTTGTAAACATAATTCAGAGTCAGTGTGGCTGCTGTCTCTTAT15.263157894736842No Hit
TTTCTAAGATGACTTGCTCTGGTGGCTGATGGTTGATGCTGGTGCAGGGA15.263157894736842No Hit
TAAACTTACAGTGCAAAGACACAACATCCACGATCATACCCCAACACCGA15.263157894736842No Hit
ACCTGCCACGAAGGGAAACCAAATCTGTTGGCCACCTTAATTTTCTATAA15.263157894736842No Hit
GGGGAAGAGTAACAAGGGTTCAGATGGAAGGTGGAGCAGAGACTAGGCTA15.263157894736842No Hit
CTCCCACTCACTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTAT15.263157894736842No Hit
TAATACAACTGTTCCATTTGATAATCAACCAATAAGAAGGTGAAAACGAA15.263157894736842No Hit
ATAAATATCATTATTATCATTATCATTTTCTTGATTGCATAAAAAATTTC15.263157894736842No Hit
AACAATAACAAGTGCAAGAGACCAATGATATTCTCGGAAACAATAAGATG15.263157894736842No Hit
TCTTTCGCTACTTGTTCCCTTGCACGCCTAGCTTCACAGAGTACCTGTAA15.263157894736842No Hit
GAGGAATATTTTTGATGTCTAATCACTAGTAGTTGAAAAAGGTACAAGGA15.263157894736842No Hit
CTATAGTTCAAAACTTACATAAATACTTTACATCCAACATTCCGATCCTC15.263157894736842No Hit
ACCTTCTAAAGAGTGGTTGTTACCGACGGAGTCACAATGAGGCTTTATTT15.263157894736842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)