FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n02_3013_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n02_3013_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132542
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC160810.7540765902852089No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT88150.41335645440980767No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG81700.38311086018469975No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT79320.37195047037760565No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC65420.30677004251264456No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT62290.2920927231444914No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG56660.2656923052394748No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA51940.24355909520187646No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC48610.22794392795077423No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT48580.22780325076833188No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC45610.21387620970653803No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT45030.2111564508459857No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC44780.20998414099229934No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT44170.20712370494930465No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC42830.2008401241335458No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG42690.20018363061548144No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA42630.19990227625059673No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC42300.1983548272437307No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG41700.19554128359488346No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT41130.1928684171284786No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT40690.1908051517859906No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG40300.1889763484142399No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG38960.18269276759848105No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC38460.18034814789110837No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT38120.17875380649009492No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC37870.17758149663640857No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG36120.16937532766060412No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA35930.16848437217180248No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG35740.16759341668300087No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC35630.16707760034737887No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA34760.16299796205655034No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG34460.16159119023212673No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC34030.1595748172837862No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT33990.1593872477071964No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGC33690.15798047588277275No Hit
CCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAG33260.15596410293443225No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG33240.15587031814613733No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA32930.15441665392756626No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA32750.15357259083291208No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT32290.1514155407021292No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC32050.1502901232425903No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC31800.14911781338890395No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT30480.14292801736144No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA30140.14133367596042656No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT30050.14091164441309947No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC29830.1398800117418555No Hit
ATCCAGCCATCTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATT29450.13809810076425225No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC29450.13809810076425225No Hit
GAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGG29390.1378167463993675No Hit
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC29380.13776985400522004No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT29340.13758228442863024No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT29310.13744160724618787No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT29220.13701957569886078No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG28700.13458117120319318No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG28680.13448738641489827No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA28390.1331275069846221No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC28110.13181451994849339No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC27900.13082977967139686No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT27850.1305953177006596No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT27750.13012639375918506No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA27350.1282506979932869No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA27300.1280162360225496No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC27290.12796934362840218No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA27270.12787555884010726No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT27010.12665635659227345No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA26500.1242648444907533No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC26170.12271739548388731No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT25940.12163887041849587No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG25710.1205603453531044No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT25520.11966938986430278No Hit
GCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGAT25450.11934114310527061No Hit
ATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCA25410.11915357352868079No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC25400.11910668113453333No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT24810.11634002987983355No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC24760.11610556790909628No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA24730.11596489072665392No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA24610.11540218199688447No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC24360.11422987214319812No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA24310.11399541017246084No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA24060.11282310031877449No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC23930.1122134991948576No Hit
GATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGT23830.11174457525338305No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC23680.11104118934117124No Hit
GAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT23620.11075983497628651No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT23540.11038469582310688No Hit
GCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC23350.10949374033430526No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG23300.10925927836356798No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT23200.10879035442209345No Hit
CAGTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATG23110.10836832287476636No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG22860.10719601302108No Hit
GTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT22770.10677398147375293No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG22760.10672708907960547No Hit
GTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT22710.1064926271088682No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT22430.10517964007273949No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC22400.10503896289029713No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT22300.10457003894882258No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAG22250.10433557697808532No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG22210.1041480074014955No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATT21920.10278812797121933No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA21620.1013813561467957No Hit
TTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGAC21600.1012875713585008No Hit
TCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAAC21450.100584185446289No Hit
CCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCC21440.10053729305214154No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAC21350.10011526150481445No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG21330.10002147671651955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)