FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n02_3013_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n02_3013_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24055
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTA10964.556225316981917No Hit
GTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGC8843.674911660777385No Hit
GGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAA7633.1718977343587613No Hit
GTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTC6402.6605695281646224No Hit
CCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCT5902.4527125337767615No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACA5452.2656412388276865No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTC4942.0536271045520684No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATT4401.829141550613178No Hit
AAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCT4261.7709415921845768No Hit
GATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGA3971.6503845354396176No Hit
CAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAG3671.5256703388069008No Hit
GGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTC3631.509041779255872No Hit
GGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAA3501.454998960715028No Hit
CAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCC3461.4383704011639993No Hit
GTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATT3011.2512991062149241No Hit
CCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGT2591.0766992309291208No Hit
ACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTA2521.0475992517148203No Hit
CACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTC2400.9977135730617336No Hit
AGCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATG2260.9395136146331324No Hit
TTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTAC2240.931199334857618No Hit
CTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAG2190.9104136354188318No Hit
CATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCA2030.8438993972147162No Hit
TAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCG1750.727499480357514No Hit
ATTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAAT1750.727499480357514No Hit
TGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTT1700.7067137809187279No Hit
AGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTC1660.690085221367699No Hit
ACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTG1610.6692995219289128No Hit
TAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCA1580.6568281022656413No Hit
TGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCA1580.6568281022656413No Hit
ATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGT1560.6485138224901268No Hit
CAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAG1560.6485138224901268No Hit
CTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTC1450.6027852837247973No Hit
GAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGA1420.5903138640615256No Hit
ACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTC1420.5903138640615256No Hit
CTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTC1420.5903138640615256No Hit
CCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAA1400.5819995842860112No Hit
ATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGAT1380.5736853045104967No Hit
ATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAA1380.5736853045104967No Hit
ACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCT1320.5487424651839534No Hit
CTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAA1300.540428185408439No Hit
GTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGTAGAGCATGTT1290.5362710455206818No Hit
TCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCT1280.5321139056329246No Hit
TCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACC1190.49469964664310956No Hit
AATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAA1190.49469964664310956No Hit
ACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGA1190.49469964664310956No Hit
CATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTG1160.48222822697983786No Hit
AGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGA1140.47391394720432345No Hit
TAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTC1130.4697568073165662No Hit
ACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACA1030.42818540843899394No Hit
AGCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAA1030.42818540843899394No Hit
ACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGA1000.41571398877572235No Hit
TTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATT980.4073997090002079No Hit
AAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAA900.3741425898981501No Hit
GTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCT880.3658283101226356No Hit
GAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAA870.3616711702348784No Hit
AAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCAC850.35335689045936397No Hit
TTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAA850.35335689045936397No Hit
GTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAA780.3242569112450634No Hit
ATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCAC750.31178549158179175No Hit
GCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGG730.3034712118062773No Hit
GCTCTCATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGAC700.2909997921430056No Hit
ACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACT690.28684265225524835No Hit
CTTTCAGACCATTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACA640.2660569528164623No Hit
CTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTG640.2660569528164623No Hit
GCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTAC630.26189981292870507No Hit
GGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGTAGAGCATGT630.26189981292870507No Hit
ACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATG620.25774267304094783No Hit
GAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACA620.25774267304094783No Hit
ACCTAGTAGAGCATGTTCCTACTTCAAATGAGAGCTTGTTGAATTTTTTT620.25774267304094783No Hit
CCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCA600.2494283932654334No Hit
GAGCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCAT600.2494283932654334No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAA590.24527125337767616No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCT590.24527125337767616No Hit
GTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTC590.24527125337767616No Hit
GCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCA570.23695697360216172No Hit
GCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAACTTGAGCGGGG560.2327998337144045No Hit
CAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACAC560.2327998337144045No Hit
AAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCAT560.2327998337144045No Hit
AGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAAC550.22864269382664726No Hit
GTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACAT540.22448555393889003No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTT530.2203284140511328No Hit
GGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAG530.2203284140511328No Hit
GAGCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCAT530.2203284140511328No Hit
CAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGT520.2161712741633756No Hit
TCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAA520.2161712741633756No Hit
CTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTC510.21201413427561835No Hit
CACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGA510.21201413427561835No Hit
GCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCT510.21201413427561835No Hit
GTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTC500.20785699438786118No Hit
CTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCTC500.20785699438786118No Hit
TGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCT500.20785699438786118No Hit
ACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGAT460.19122843483683227No Hit
GGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTAC450.18707129494907504No Hit
TCACACCACTGTGCTCCAGCCTGGTGATAGAGCGAGACTCCAACAAAAAA440.1829141550613178No Hit
GTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGT440.1829141550613178No Hit
GATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGAA430.1787570151735606No Hit
CACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTT430.1787570151735606No Hit
AGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATC410.17044273539804614No Hit
CTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTC410.17044273539804614No Hit
AGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGG410.17044273539804614No Hit
CTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCT410.17044273539804614No Hit
GCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACA400.1662855955102889No Hit
AAGCAGGAGAATGGCGCGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGG390.1621284556225317No Hit
CATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATT390.1621284556225317No Hit
ATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGAACA380.1579713157347745No Hit
AGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCA370.15381417584701726No Hit
AACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAGCTGC370.15381417584701726No Hit
CTCATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTT370.15381417584701726No Hit
GCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTC370.15381417584701726No Hit
GCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCAC370.15381417584701726No Hit
CAGGAGAACCGCTTGAACCAGGGAGCTGGAGGTTGCAGTGAGCCGAGATC360.14965703595926003No Hit
AAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACA360.14965703595926003No Hit
TACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAG360.14965703595926003No Hit
AACTAATTCCGCAGAGATGAACTTGAGCGGGGAGCAGAGGAAAAGGTACG360.14965703595926003No Hit
GTCTGAAAGTCCACATCATGTTTACCTAGTAGAGCATGTTCCTACTTCAA360.14965703595926003No Hit
CTCTCATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACT350.1454998960715028No Hit
GAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCA340.1413427561837456No Hit
GATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAGCTGCTGTGG340.1413427561837456No Hit
TCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGT330.13718561629598836No Hit
GCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAA330.13718561629598836No Hit
CTCCTGCCTCAGCCTCTCCGAGTAGCTGGGACTACAGGCGCCCGCCGCCA330.13718561629598836No Hit
GGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTAC330.13718561629598836No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAAAATTCAACAAGCTCTCAT320.13302847640823115No Hit
GCCTCAGCCTCTCCGAGTAGCTGGGACTACAGGCGCCCGCCGCCACGCCC320.13302847640823115No Hit
CTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCC320.13302847640823115No Hit
ACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAG310.12887133652047392No Hit
CAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATG310.12887133652047392No Hit
TGTTTACCTAGTAGAGCATGTTCCTACTTCAAATGAGAGCTTGTTGAATT310.12887133652047392No Hit
CAGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAA310.12887133652047392No Hit
GATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAACT300.1247141966327167No Hit
CCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAG300.1247141966327167No Hit
GTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAGC290.12055705674495946No Hit
GCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAA290.12055705674495946No Hit
CAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTA290.12055705674495946No Hit
CAACAAGCTCTCATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGAT290.12055705674495946No Hit
GTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGTAGAG280.11639991685720225No Hit
GCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAAC280.11639991685720225No Hit
CCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGAT270.11224277696944501No Hit
TACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTAT270.11224277696944501No Hit
CTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACC270.11224277696944501No Hit
CTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCC270.11224277696944501No Hit
CTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTG270.11224277696944501No Hit
GGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTA260.1080856370816878No Hit
CAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATG260.1080856370816878No Hit
TTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTG260.1080856370816878No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAAATTCAAC260.1080856370816878No Hit
GACTACAGGCGCCCGCCGCCACGCCCGGCTAATTTTTTGTATTTTTAGTA260.1080856370816878No Hit
GGGTTTTACACATAACTAATTCCGCAGAGATGAACTTGAGCGGGGAGCAG260.1080856370816878No Hit
TTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTA250.10392849719393059No Hit
CGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGT250.10392849719393059No Hit
TAACTAATTCCGCAGAGATGAACTTGAGCGGGGAGCAGAGGAAAAGGTAC250.10392849719393059No Hit
ACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAGCTGCT250.10392849719393059No Hit
GCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGG250.10392849719393059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)