FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n02_3012_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n02_3012_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24586
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA14986.09289839746197No Hit
TCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGA12214.966240950134223No Hit
TCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCG10314.193443423086309No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG10054.08769218254291No Hit
GTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAA8453.436915317660457No Hit
GTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCT7833.184739282518506No Hit
GTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCG6342.5787033270967217No Hit
AAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGA5272.143496298706581No Hit
AAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTT4922.0011388595135444No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC4651.8913202635646305No Hit
GGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTT4451.8099731554543235No Hit
TCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGA4221.7164239811274709No Hit
AATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAA3821.5537297649068575No Hit
CTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCG3781.5374603432847962No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG3711.508988855446189No Hit
GCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGG3081.2527454648987228No Hit
AGTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTC2761.1225900919222322No Hit
GTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGA2641.0737818270560482No Hit
AGCGAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAA2541.0331082730008947No Hit
GCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTA2491.012771495973318No Hit
CCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATG2370.9639632311071341No Hit
GTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGAC2260.9192223216464654No Hit
GAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCG2210.8988855446188888No Hit
GAGCAAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGA2130.8663467013747662No Hit
TTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAA2080.8460099243471895No Hit
ATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGT2010.8175384365085822No Hit
CTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCT1850.7524607500203367No Hit
GGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAAT1850.7524607500203367No Hit
CTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTC1820.7402586838037908No Hit
GTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAA1750.7117871959651834No Hit
CATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGA1710.6955177743431221No Hit
GGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGA1470.5979012446107541No Hit
TCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCAT1460.5938338892052387No Hit
TTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGG1440.5856991783942082No Hit
GAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGA1430.5816318229886928No Hit
AAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT1370.5572276905556007No Hit
TCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGA1360.5531603351500854No Hit
ACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGA1340.5450256243390548No Hit
GTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCA1290.524688847311478No Hit
ACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGATGATGGA1240.5043520702839014No Hit
ACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCG1200.48808264866184004No Hit
GCAAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAA1130.4596111608232327No Hit
CAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGA1130.4596111608232327No Hit
ACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACAT1120.45554380541771744No Hit
CATCTGACATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCA1000.4067355405515334No Hit
GAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAAT980.3986008297405027No Hit
CCTCTGCATTGTCCCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG980.3986008297405027No Hit
GAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCG930.37826405271292607No Hit
CTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAA880.3579272756853494No Hit
CACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGG870.35385992027983404No Hit
ATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTG860.3497925648743187No Hit
ATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTCCCCGCCCCTG860.3497925648743187No Hit
AGCAAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAA850.3457252094688034No Hit
GTCATATTCCTCTGCATTGTCCCCGAAGAAATAAGATCCTTCATTACTCA830.3375904986577727No Hit
ATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTAC810.329455787846742No Hit
GGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGATG760.3091190108191654No Hit
GCGAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAA740.3009843000081347No Hit
CGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATC740.3009843000081347No Hit
GGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTC740.3009843000081347No Hit
GGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGAT740.3009843000081347No Hit
CCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAATACCGAGGG730.29691694460261936No Hit
AGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAA720.2928495891971041No Hit
ATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATT710.2887822337915887No Hit
GACATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCAGAAGA700.28471487838607334No Hit
TTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTG700.28471487838607334No Hit
AATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAATAC700.28471487838607334No Hit
CTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCA690.28064752298055806No Hit
CATAAGGATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGGGGC690.28064752298055806No Hit
GGGCAGAACATCTGACATGAGGACTGAAATCATAAGGATGATGGAAAGTG690.28064752298055806No Hit
CAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATT680.2765801675750427No Hit
GGATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGGGGCGGGGA660.26844545676401205No Hit
TTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACT650.2643781013584967No Hit
CTCGAAGACTCCCCGCCCCTGGAAAGACACATCTTCTGGTCTGGCACTTT630.25624339054746603No Hit
TTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGA630.25624339054746603No Hit
TTCCAGGGGCGGGGAGTCTTCGAGCTCTCGGACGAAAAGGCAACGAACCC620.2521760351419507No Hit
GGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATA620.2521760351419507No Hit
CCCCTGGAAAGACACATCTTCTGGTCTGGCACTTTCCATCATCCTTATGA610.24810867973643538No Hit
TCTGACATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCAGA610.24810867973643538No Hit
GACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTC580.23590661351988937No Hit
TTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCAT570.23183925811437403No Hit
TTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGAT520.21150248108679737No Hit
ACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGG480.19523305946473604No Hit
ATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAG470.19116570405922068No Hit
GCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAAT460.18709834865370537No Hit
GACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACA450.18303099324819003No Hit
ATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTT450.18303099324819003No Hit
TCATAAGGATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGGGG450.18303099324819003No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTATTTTTCTTCAATTGT450.18303099324819003No Hit
AATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTT440.1789636378426747No Hit
GAACATCTGACATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGA440.1789636378426747No Hit
GTGCCAGACCAGAAGATGTGTCTTTCCAGGGGCGGGGAGTCTTCGAGCTC440.1789636378426747No Hit
GTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCC430.17489628243715935No Hit
GGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTCCCCGCCCCTGGAAA420.170828927031644No Hit
TCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGAT410.1667615716261287No Hit
ATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCA400.16269421622061336No Hit
GAGCGAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGA400.16269421622061336No Hit
CATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCAGAAGATG400.16269421622061336No Hit
GGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCAGAAGATGTGTCT380.1545595054095827No Hit
CTCCCCGCCCCTGGAAAGACACATCTTCTGGTCTGGCACTTTCCATCATC380.1545595054095827No Hit
CCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTC380.1545595054095827No Hit
CTTCTGGTCTGGCACTTTCCATCATCCTTATGATTTCAGTCCTCATGTCA380.1545595054095827No Hit
GATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTT370.15049215000406735No Hit
GACCAGAAGATGTGTCTTTCCAGGGGCGGGGAGTCTTCGAGCTCTCGGAC360.14642479459855204No Hit
ATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGA360.14642479459855204No Hit
AACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAA360.14642479459855204No Hit
TAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGT350.14235743919303667No Hit
TTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCT350.14235743919303667No Hit
CCGACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGA350.14235743919303667No Hit
CTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATC340.13829008378752136No Hit
CTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGG330.13422272838200602No Hit
GACCGACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCT330.13422272838200602No Hit
GAAGATGTGTCTTTCCAGGGGCGGGGAGTCTTCGAGCTCTCGGACGAAAA320.13015537297649069No Hit
GGTCTGGCACTTTCCATCATCCTTATGATTTCAGTCCTCATGTCAGATGT320.13015537297649069No Hit
ACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCA320.13015537297649069No Hit
TATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCAT300.12202066216546001No Hit
ATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGATGATG300.12202066216546001No Hit
CAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCC290.11795330675994468No Hit
GTCCCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGA290.11795330675994468No Hit
CCAGAAGATGTGTCTTTCCAGGGGCGGGGAGTCTTCGAGCTCTCGGACGA280.11388595135442936No Hit
ATCATAAGGATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGGG270.10981859594891401No Hit
CCTTTGACATGAGTAATGAAGGATCTTATTTCTTCGGAGACAATGCAGAG260.10575124054339868No Hit
AGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGG250.10168388513788335No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)