FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n02_3010_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n02_3010_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42500
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTA32097.550588235294117No Hit
GTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTC19594.609411764705882No Hit
GGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAA19044.4799999999999995No Hit
GTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGC17994.232941176470588No Hit
AAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCT13863.2611764705882353No Hit
CCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCT13443.1623529411764704No Hit
GGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAA11982.8188235294117647No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACA11652.7411764705882353No Hit
GGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTC10922.5694117647058823No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATT9532.242352941176471No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTC9462.2258823529411766No Hit
CAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCC8912.0964705882352943No Hit
CAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAG7391.7388235294117649No Hit
GATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGA7191.691764705882353No Hit
AGCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATG5751.352941176470588No Hit
TTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTAC5201.223529411764706No Hit
AGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTC5161.2141176470588235No Hit
TAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCA4731.1129411764705883No Hit
ACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTA4681.1011764705882352No Hit
GTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATT4541.0682352941176472No Hit
ATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGAT4210.9905882352941175No Hit
CACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTC4070.9576470588235294No Hit
CTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTC3700.8705882352941177No Hit
ACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTC3430.8070588235294117No Hit
TGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTT3420.8047058823529413No Hit
ATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAA3250.7647058823529412No Hit
CCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGT3170.7458823529411766No Hit
ACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTG3000.7058823529411765No Hit
AATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAA2980.7011764705882353No Hit
ACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCT2930.6894117647058823No Hit
TAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTC2900.6823529411764706No Hit
TGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCA2870.6752941176470588No Hit
CAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAG2840.668235294117647No Hit
TAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCG2770.6517647058823529No Hit
AGCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAA2740.6447058823529411No Hit
TCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACC2630.6188235294117648No Hit
CTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTC2600.611764705882353No Hit
GAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAA2510.5905882352941176No Hit
CATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTG2340.5505882352941176No Hit
CATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCA2330.548235294117647No Hit
GCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGG2300.5411764705882353No Hit
AAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAA2300.5411764705882353No Hit
CCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAA2300.5411764705882353No Hit
TCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCT2210.52No Hit
AAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCAC2180.5129411764705882No Hit
GAGCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCAT2070.48705882352941177No Hit
CTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAG2010.4729411764705882No Hit
GAGCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCAT2000.4705882352941176No Hit
ACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGA1850.43529411764705883No Hit
CTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTG1800.4235294117647058No Hit
CAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACAC1720.40470588235294114No Hit
CTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAA1710.40235294117647064No Hit
GCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACA1680.3952941176470588No Hit
ACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACT1650.38823529411764707No Hit
GTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACAT1530.36No Hit
TCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAA1490.35058823529411764No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAA1470.3458823529411765No Hit
CTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCT1420.3341176470588235No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCT1340.31529411764705884No Hit
GAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGA1330.3129411764705882No Hit
CCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCA1310.30823529411764705No Hit
ATTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAAT1290.3035294117647059No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTT1250.29411764705882354No Hit
TTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAA1230.28941176470588237No Hit
CATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATT1180.2776470588235294No Hit
ATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGT1140.26823529411764707No Hit
AGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCA1130.26588235294117646No Hit
AGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGA1070.25176470588235295No Hit
GCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCC1070.25176470588235295No Hit
CACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTT1050.24705882352941178No Hit
ACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACA1010.2376470588235294No Hit
CAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATG970.22823529411764706No Hit
GCTCTCATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGAC920.2164705882352941No Hit
ATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGAACA910.21411764705882352No Hit
AAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACA910.21411764705882352No Hit
GCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAA900.2117647058823529No Hit
CACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGA900.2117647058823529No Hit
GATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGAA890.20941176470588235No Hit
AAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAG880.20705882352941174No Hit
GCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAA870.20470588235294118No Hit
GTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGTAGAGCATGTT840.1976470588235294No Hit
CTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTC830.19529411764705884No Hit
TGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCT820.19294117647058823No Hit
GAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTC810.19058823529411764No Hit
GCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGG800.18823529411764706No Hit
AGCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATG790.18588235294117647No Hit
CGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGT780.18352941176470589No Hit
GTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCT760.17882352941176471No Hit
GGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTA760.17882352941176471No Hit
ACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATG760.17882352941176471No Hit
CAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGT760.17882352941176471No Hit
GCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAAC750.17647058823529413No Hit
GTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAA740.17411764705882354No Hit
ATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCAC740.17411764705882354No Hit
GCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCT710.16705882352941176No Hit
GTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTC690.1623529411764706No Hit
GGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTAC670.15764705882352942No Hit
GCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCAC650.15294117647058825No Hit
GAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACA640.15058823529411766No Hit
CTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTC610.1435294117647059No Hit
GCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTAC610.1435294117647059No Hit
TTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATT600.1411764705882353No Hit
AAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCAT590.1388235294117647No Hit
AGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGG580.13647058823529412No Hit
GTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTC580.13647058823529412No Hit
TTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTT560.13176470588235295No Hit
GAGCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGA560.13176470588235295No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAAATTCAAC540.12705882352941178No Hit
GAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACG540.12705882352941178No Hit
ACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGAT540.12705882352941178No Hit
GCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTC530.1247058823529412No Hit
ACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGA530.1247058823529412No Hit
CTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGT520.12235294117647058No Hit
TTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTG510.12No Hit
GAGCAAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGA510.12No Hit
GCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCA500.1176470588235294No Hit
CTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAA500.1176470588235294No Hit
TACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAG500.1176470588235294No Hit
GCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCT490.11529411764705881No Hit
ACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAG490.11529411764705881No Hit
CTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAG480.11294117647058823No Hit
AACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAAC470.11058823529411764No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAAAATTCAACAAGCTCTCAT460.10823529411764705No Hit
TACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTAT450.10588235294117646No Hit
AGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATC440.10352941176470587No Hit
TCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGT430.10117647058823528No Hit
TGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAA430.10117647058823528No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)