FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n02_3000_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n02_3000_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908565
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA137080.4712977017876513No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG132640.45603244211492605No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG106900.36753519347169483No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT79260.27250551388743244No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG76360.2625349613984903No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC72420.24898876249972063No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA71040.24424415476360334No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG66550.2288069890134826No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG59410.20425880116139747No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT57760.19858590060734416No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT47930.16478916579137823No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG47560.1635170608186511No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC45630.15688148623118273No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA43100.14818303871496769No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT42420.14584511606238815No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG42020.1444698674432237No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC40480.13917516025944066No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA40170.1381093425795882No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG39360.13532446412578025No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT37180.12782935915133406No Hit
TTTTTGGACAGTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAA35660.12260341439850923No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA34930.12009358566853413No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT34750.11947472378991014No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC34300.11792756909335016No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC33670.11576155251816617No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA32600.11208276246190133No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG31240.10740691715674223No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC30710.10558471273634937No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATG30490.10482832599580892No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG30110.10352183980760271No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG29720.1021809724039174No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC29650.10194030389556362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)