FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n02_2858_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n02_2858_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345554
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG98170.4185365163198119No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT75850.3233777606484438No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA51610.220033305564485No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG50040.21333979094064773No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA49030.20903377197881606No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT47410.20212708809944263No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA47210.20127441107729774No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG43630.18601149238090448No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT41160.17548093115741528No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG36910.15736154443683667No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC36030.15360976553939923No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG34820.14845106955542273No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA33430.14252496425151584No Hit
GTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGCGTTTCCTGGG32830.13996693318508124No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT32580.13890108690740013No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA32340.13787787448082628No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT31990.13638568969207274No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA31630.13485087105221197No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG30000.12790155332173125No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACATGTTGGGAACAA29660.12645200238408497No Hit
GTATACATTGTGGTCATAAGTTCCATTCCTGATTGACCCCATGCAGGCAT29580.12611093157522701No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA29310.12495981759533142No Hit
GTCCTGAATTCTCCCTTCTACTTCTGAGAACTCTTTTTCAATCTGATGGA29240.12466138063758073No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA28760.12261495578443302No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC28560.12176227876228814No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA28180.12014219242021287No Hit
ATATAAGGTTCTTTCCATCCAAAGAATGTTTTTATGCACTTGATCGCATC27540.11741362594934927No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC27380.11673148433163338No Hit
CTCAGTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC27150.11575090575616678No Hit
GTATTATTATCTCCTGAGGAAGTCAGTGAAACACAGGGAACTGAAAGACT26390.11251073307201624No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT25940.1105922097721903No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG25850.11020850511222507No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA25790.10995270200558163No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT25070.10688306472586007No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC24710.10534824608599931No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC24670.10517771068157032No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC24550.1046661044682834No Hit
CCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT24330.10372815974392403No Hit
ACCTTATATAGTCAAACCACACGAAAAGGGAATAAATTCAAATTACCTGC24090.10270494731735018No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA24030.10244914421070674No Hit
TGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACGATCAAGAATCCA23830.10159646718856186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)