FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n02_2855_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n02_2855_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255453
Sequences flagged as poor quality0
Sequence length151
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGC8070.31590938450517314No Hit
GCTTTCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7470.2924216979248629No Hit
CCCCACGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGC6890.269716934230563No Hit
GAGCAAAAGCAGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCG4670.18281249388341494No Hit
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGAGCGAAAGCAG4660.1824210324404098No Hit
GCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAATAACCCGGGGGG4430.17341741925129084No Hit
CTAATAACCCGTGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCC4260.16676257472020292No Hit
CCCGTGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGG4020.15736750008807884No Hit
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAAT3860.15110411699999607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3710.14523219535491852No Hit
GGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCTGCTTTC3670.14366634958289784No Hit
GCTTTCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3620.14170904236787196No Hit
GAGCAAAAGCAGGGGGGAGCAAAAGCAGCCTTGTTTCTACTAATAACCCG3490.13662004360880475No Hit
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGTGGGGGGAGCGAAAGC3220.1260505846476651No Hit
CCCGTGGGGGGAGCGAAAGCAGGCCTTGTTTCTACTAATAACCCGGGGGG3030.11861281723056687No Hit
CCCGTGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGA2790.10921774259844276No Hit
GCTTTCGCTCCCCCCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAG2700.10569458961139622No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)