FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n01_Neg_Water_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n01_Neg_Water_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTACTTCCATTATATTAATTTTTTTTATTAAGAAATAATAATTTCTAG15.263157894736842No Hit
TCTAATTATCCTTACCCTCTCCAAACAGGACCAGTTACCTTGAATTCATG15.263157894736842No Hit
AGCAAAAGGAAGTCCTATATAAATAAAAAAGGTCCATTAGAAGTCACTAT15.263157894736842No Hit
GTTTAAATAAATGTATCTGTAATATATTCGTAATTTACTCCCCTCTCGGG15.263157894736842No Hit
CTTGAAAATTGTTGCACAGTCGAGGCTCCTTTGAATTCCTTGTACCTTTT15.263157894736842No Hit
AGGCAAACTCTTACATTCCAGCTTAACAGAGTTCTTCGCAGAACTGAGGT15.263157894736842No Hit
ATCTACGAGAGGTGTTTTGAATTTCTCCTTTTATAGTTACATGCGAACTA15.263157894736842No Hit
AGCTTTTGCAAAGTGATATATAACATGTATCGAGAGTGACATTTAAGCAT15.263157894736842No Hit
TTATATAGTGATGCAAGAGCATGGGGGTTGAAGACAAAAGGCTTCATTTT15.263157894736842No Hit
GGCTCCAAGCAAACAAAAGCAAACAAATAAACAAAACTACATTAAAAATA15.263157894736842No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA15.263157894736842No Hit
CACCTGTTAGGATGGCTAACAGCGAGCACACAAAAACTGATCGCCAGCGC15.263157894736842No Hit
CACTTAACCAACTGTCTAGGTGCCCCATTATTTTTTCATTTCATATCTTT15.263157894736842No Hit
CAGCCACACTGACTCTGAATTATGTTTACAAACTAAATACTGTCTCTTAT15.263157894736842No Hit
GAATAGACCCTTTCAAACTACTCCAAAATAGCAAAATATACAGCTTAATC15.263157894736842No Hit
ATCAATAAAAGCTATTCAAGTTTTCCTCCATACTCTTGTGTCATTTATGT15.263157894736842No Hit
GTGCGGAGCCAGAGGCTCGGTTCAGTGGCCAGAGCACCACACTGGCGCTC15.263157894736842No Hit
ATCTGGATTCCTCGCAGAGCAGAGGGGCCAAGTCTGTTTCATGGAACATG15.263157894736842No Hit
GCTCCATAGTATGGCAGGACGATAACGTCTGACTACTGTTGGAAGTCCTG15.263157894736842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)