FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n01_3013_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n01_3013_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24055
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTA9724.040739970900021No Hit
GTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGC8583.5668260236956977No Hit
GGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAA7473.1053834961546456No Hit
GTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTC6732.797755144460611No Hit
CCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCT6502.702140927042195No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATT5182.1533984618582416No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTC5182.1533984618582416No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACA5172.1492413219704845No Hit
AAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCT4631.924755768031594No Hit
GATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGA3951.6420702556641031No Hit
GGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTC3721.5464560382456871No Hit
CAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAG3611.5007274994803577No Hit
GGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAA3591.4924132197048432No Hit
CAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCC3031.2596133859904386No Hit
GTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATT2921.2138848472251091No Hit
ACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTA2781.1556848887965079No Hit
CACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTC2411.0018707129494908No Hit
CTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAG2320.9644564539596757No Hit
CCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGT2310.9602993140719185No Hit
AGCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATG2140.8896279359800456No Hit
CAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAG2020.839742257326959No Hit
TTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTAC1980.8231136977759302No Hit
CATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCA1880.781542298898358No Hit
AGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTC1800.7482851797963002No Hit
CTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTC1750.727499480357514No Hit
ACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTC1640.6817709415921845No Hit
ATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAA1610.6692995219289128No Hit
TGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTT1590.6609852421533984No Hit
GAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGA1590.6609852421533984No Hit
AATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAA1550.6443566826023696No Hit
ACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTG1550.6443566826023696No Hit
CTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTC1510.6277281230513407No Hit
ATTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAAT1510.6277281230513407No Hit
TAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCA1500.6235709831635835No Hit
CATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTG1400.5819995842860112No Hit
TGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCA1400.5819995842860112No Hit
CCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAA1390.5778424443982539No Hit
TAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCG1390.5778424443982539No Hit
TCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACC1350.5612138848472251No Hit
ATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGT1320.5487424651839534No Hit
CTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAA1290.5362710455206818No Hit
ACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCT1280.5321139056329246No Hit
TCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCT1270.5279567657451674No Hit
ATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGAT1240.5154853460818957No Hit
ACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGA1200.4988567865308668No Hit
TAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTC1140.47391394720432345No Hit
GAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAA1060.4406568281022656No Hit
GTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGTAGAGCATGTT1040.4323425483267512No Hit
AGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGA1020.4240282685512367No Hit
AGCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAA970.40324256911245066No Hit
ACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGA960.3990854292246934No Hit
AAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAA890.36998545001039285No Hit
ACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACA870.3616711702348784No Hit
CTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTG860.3575140303471212No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAA840.34919975057160674No Hit
TCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAA810.33672833090833504No Hit
AAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCAC790.32841405113282063No Hit
TTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAA740.3076283516940345No Hit
ACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGAT720.29931407191852005No Hit
GTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAA710.2951569320307628No Hit
GTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCT710.2951569320307628No Hit
GAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACA700.2909997921430056No Hit
CAGGAGAACCGCTTGAACCAGGGAGCTGGAGGTTGCAGTGAGCCGAGATC690.28684265225524835No Hit
CCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCA690.28684265225524835No Hit
ATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCAC680.2826855123674912No Hit
GAGCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCAT640.2660569528164623No Hit
TTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATT630.26189981292870507No Hit
GCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGG620.25774267304094783No Hit
ACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATG610.2535855331531906No Hit
GGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTAC610.2535855331531906No Hit
GTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACAT600.2494283932654334No Hit
CATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATT600.2494283932654334No Hit
GCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTAC600.2494283932654334No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTT600.2494283932654334No Hit
AGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAAC600.2494283932654334No Hit
GCTCTCATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGAC590.24527125337767616No Hit
GCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAACTTGAGCGGGG590.24527125337767616No Hit
ACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACT580.24111411348991893No Hit
CTTTCAGACCATTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACA570.23695697360216172No Hit
GTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTC570.23695697360216172No Hit
GGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAG540.22448555393889003No Hit
CAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGT540.22448555393889003No Hit
AAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCAT520.2161712741633756No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCT520.2161712741633756No Hit
GTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTC520.2161712741633756No Hit
GCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACA500.20785699438786118No Hit
GAGCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCAT500.20785699438786118No Hit
CTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTC490.20369985450010394No Hit
AGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCA490.20369985450010394No Hit
GCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCT490.20369985450010394No Hit
GGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGTAGAGCATGT490.20369985450010394No Hit
TGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCT480.1995427146123467No Hit
ATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGAACA480.1995427146123467No Hit
GATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGAA480.1995427146123467No Hit
CACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTT470.1953855747245895No Hit
ACCTAGTAGAGCATGTTCCTACTTCAAATGAGAGCTTGTTGAATTTTTTT460.19122843483683227No Hit
CTCATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTT450.18707129494907504No Hit
CTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCTC450.18707129494907504No Hit
CTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCT440.1829141550613178No Hit
TCACACCACTGTGCTCCAGCCTGGTGATAGAGCGAGACTCCAACAAAAAA430.1787570151735606No Hit
TACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAG430.1787570151735606No Hit
CAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACAC430.1787570151735606No Hit
TCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGT410.17044273539804614No Hit
GCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCAC410.17044273539804614No Hit
GATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAGCTGCTGTGG410.17044273539804614No Hit
CACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGA390.1621284556225317No Hit
GTCTGAAAGTCCACATCATGTTTACCTAGTAGAGCATGTTCCTACTTCAA390.1621284556225317No Hit
GCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAA380.1579713157347745No Hit
GCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCA380.1579713157347745No Hit
AGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGG370.15381417584701726No Hit
ACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAG360.14965703595926003No Hit
AAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAG360.14965703595926003No Hit
CTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGT360.14965703595926003No Hit
TAACTAATTCCGCAGAGATGAACTTGAGCGGGGAGCAGAGGAAAAGGTAC350.1454998960715028No Hit
AGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATC330.13718561629598836No Hit
GTTTTACACATAACTAATTCCGCAGAGATGAACTTGAGCGGGGAGCAGAG330.13718561629598836No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAAATTCAAC330.13718561629598836No Hit
GAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCA330.13718561629598836No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAAAATTCAACAAGCT330.13718561629598836No Hit
CTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCC320.13302847640823115No Hit
AACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAGCTGC320.13302847640823115No Hit
GATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAACT310.12887133652047392No Hit
AACTAATTCCGCAGAGATGAACTTGAGCGGGGAGCAGAGGAAAAGGTACG310.12887133652047392No Hit
GCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTC300.1247141966327167No Hit
GGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTA300.1247141966327167No Hit
GCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAAC300.1247141966327167No Hit
TTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTT300.1247141966327167No Hit
ACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCA290.12055705674495946No Hit
GCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAA290.12055705674495946No Hit
GAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAACTTGAG290.12055705674495946No Hit
ACTGTGCTCCAGCCTGGTGATAGAGCGAGACTCCAACAAAAAAAAGAAAA290.12055705674495946No Hit
CTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTC290.12055705674495946No Hit
CTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTA290.12055705674495946No Hit
GTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGT290.12055705674495946No Hit
AAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACA290.12055705674495946No Hit
TACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTAT290.12055705674495946No Hit
AAGCAGGAGAATGGCGCGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGG280.11639991685720225No Hit
GTGATAGAGCGAGACTCCAACAAAAAAAAGAAAAAAAAGGCCGGGCGCGG280.11639991685720225No Hit
TGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACC280.11639991685720225No Hit
TTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTG270.11224277696944501No Hit
GGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTAC270.11224277696944501No Hit
ACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAGCTGCT270.11224277696944501No Hit
ACACATAACTAATTCCGCAGAGATGAACTTGAGCGGGGAGCAGAGGAAAA270.11224277696944501No Hit
CTCCTGCCTCAGCCTCTCCGAGTAGCTGGGACTACAGGCGCCCGCCGCCA270.11224277696944501No Hit
CAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATG260.1080856370816878No Hit
CGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGT260.1080856370816878No Hit
GCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCC260.1080856370816878No Hit
CCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAG260.1080856370816878No Hit
ATCTCGGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGC250.10392849719393059No Hit
AGCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATG250.10392849719393059No Hit
GTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGTAGAGCAT250.10392849719393059No Hit
CTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTG250.10392849719393059No Hit
CAGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAA250.10392849719393059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)