FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n01_3012_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n01_3012_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24586
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA15716.38981534206459No Hit
TCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGA12315.006914504189377No Hit
TCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCG10174.1365004474090945No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG9603.9046611892947203No Hit
GTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAA8903.619946310908647No Hit
GTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCT7973.2416822581957208No Hit
GTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCG6512.6478483689904824No Hit
AAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGA5862.383470267631986No Hit
GGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTT5402.19637191897828No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC5022.0418124135686977No Hit
AAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTT5012.0377450581631824No Hit
CTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCG4251.728626047344017No Hit
TCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGA4221.7164239811274709No Hit
AATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAA3831.5577971203123728No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG3551.4439111689579436No Hit
GCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGG3401.3829008378752137No Hit
AGTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTC3201.301553729764907No Hit
GTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGA2621.0656471162450176No Hit
AGCGAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAA2591.0534450500284716No Hit
GTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGAC2420.9843000081347109No Hit
CCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATG2380.9680305865126495No Hit
GCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTA2140.8704140567802814No Hit
TTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAA1970.8012690148865208No Hit
GGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAAT1960.7972016594810054No Hit
CTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTC1910.7768648824534288No Hit
GAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCG1900.7727975270479135No Hit
GAGCAAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGA1900.7727975270479135No Hit
ATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGT1870.7605954608313674No Hit
GTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAA1850.7524607500203367No Hit
CATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGA1640.6670462865045148No Hit
TCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCAT1580.6426421540714228No Hit
TTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGG1500.6101033108273002No Hit
ACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGA1370.5572276905556007No Hit
ACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGATGATGGA1320.5368909135280241No Hit
CAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGA1310.5328235581225088No Hit
CTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCT1290.524688847311478No Hit
GAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGA1240.5043520702839014No Hit
TCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGA1210.49215000406735543No Hit
ACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCG1200.48808264866184004No Hit
GGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGA1170.475880582445294No Hit
ACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACAT1150.46774587163426345No Hit
GTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCA1070.4352070283901407No Hit
CATCTGACATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCA1050.42707231757911013No Hit
GAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAAT1030.4189376067680794No Hit
GAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCG990.4026681851460181No Hit
ATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTCCCCGCCCCTG980.3986008297405027No Hit
GCAAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAA960.3904661189294721No Hit
AAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT960.3904661189294721No Hit
GACATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCAGAAGA940.38233140811844135No Hit
GTCATATTCCTCTGCATTGTCCCCGAAGAAATAAGATCCTTCATTACTCA920.37419669730741073No Hit
CCTCTGCATTGTCCCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG890.3619946310908647No Hit
ATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTG880.3579272756853494No Hit
CACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGG850.3457252094688034No Hit
AGCAAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAA840.341657854063288No Hit
ATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATT830.3375904986577727No Hit
GGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGATG820.3335231432522574No Hit
CGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATC810.329455787846742No Hit
CTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAA770.3131863662246807No Hit
GGGCAGAACATCTGACATGAGGACTGAAATCATAAGGATGATGGAAAGTG750.3050516554136501No Hit
ATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTAC740.3009843000081347No Hit
CATAAGGATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGGGGC730.29691694460261936No Hit
GGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATA710.2887822337915887No Hit
AGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAA690.28064752298055806No Hit
GGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGAT650.2643781013584967No Hit
TTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTG640.26031074595298137No Hit
GCGAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAA630.25624339054746603No Hit
TTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACT630.25624339054746603No Hit
CAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATT630.25624339054746603No Hit
CCCCTGGAAAGACACATCTTCTGGTCTGGCACTTTCCATCATCCTTATGA620.2521760351419507No Hit
GGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTC620.2521760351419507No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGGTATTTTTCTTCAATTGT610.24810867973643538No Hit
GTGCCAGACCAGAAGATGTGTCTTTCCAGGGGCGGGGAGTCTTCGAGCTC570.23183925811437403No Hit
GCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAAT570.23183925811437403No Hit
TTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCAT560.22777190270885872No Hit
TCTGACATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCAGA560.22777190270885872No Hit
AATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTT550.22370454730334335No Hit
AATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAATAC550.22370454730334335No Hit
CCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAATACCGAGGG530.2155698364923127No Hit
GAGCGAAAGCAGGACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGA520.21150248108679737No Hit
CTCGAAGACTCCCCGCCCCTGGAAAGACACATCTTCTGGTCTGGCACTTT510.20743512568128206No Hit
TCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGAT510.20743512568128206No Hit
ATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTT500.2033677702757667No Hit
GACAAATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTC500.2033677702757667No Hit
TTCCAGGGGCGGGGAGTCTTCGAGCTCTCGGACGAAAAGGCAACGAACCC500.2033677702757667No Hit
TTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGA490.19930041487025135No Hit
GAACATCTGACATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGA480.19523305946473604No Hit
GTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCC460.18709834865370537No Hit
CTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCA450.18303099324819003No Hit
GGATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGGGGCGGGGA450.18303099324819003No Hit
ATACCGAGGGCAGAACATCTGACATGAGGACTGAAATCATAAGGATGATG440.1789636378426747No Hit
AACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAA440.1789636378426747No Hit
GATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTT430.17489628243715935No Hit
CTTCTGGTCTGGCACTTTCCATCATCCTTATGATTTCAGTCCTCATGTCA420.170828927031644No Hit
ACAGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGG410.1667615716261287No Hit
ATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCA400.16269421622061336No Hit
TTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGAT400.16269421622061336No Hit
CATGAGGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCAGAAGATG390.15862686081509803No Hit
ACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCA380.1545595054095827No Hit
TCATAAGGATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGGGG380.1545595054095827No Hit
TTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCT370.15049215000406735No Hit
GAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGT370.15049215000406735No Hit
GGACTGAAATCATAAGGATGATGGAAAGTGCCAGACCAGAAGATGTGTCT360.14642479459855204No Hit
AGAGAAATCTTCCCTTCGAAAGACCGACCATTATGGCTGCGTTTAAGGGG350.14235743919303667No Hit
CATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATG350.14235743919303667No Hit
GACCATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACA340.13829008378752136No Hit
CTCCCCGCCCCTGGAAAGACACATCTTCTGGTCTGGCACTTTCCATCATC340.13829008378752136No Hit
ATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGA340.13829008378752136No Hit
TATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCAT320.13015537297649069No Hit
GACCAGAAGATGTGTCTTTCCAGGGGCGGGGAGTCTTCGAGCTCTCGGAC320.13015537297649069No Hit
ATTATGGCTGCGTTTAAGGGGAATACCGAGGGCAGAACATCTGACATGAG320.13015537297649069No Hit
CTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGG310.12608801757097535No Hit
GATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGGGGCGGGGAG310.12608801757097535No Hit
AAACAAGGGTATTTTTCTTCAATTGTCATATTCCTCTGCATTGTCCCCGA300.12202066216546001No Hit
GCATTGTCCCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGG300.12202066216546001No Hit
TCCTTATGATTTCAGTCCTCATGTCAGATGTTCTGCCCTCGGTATTCCCC300.12202066216546001No Hit
ATCCTTATGATTTCAGTCCTCATGTCAGATGTTCTGCCCTCGGTATTCCC270.10981859594891401No Hit
GAAATCATAAGGATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCA270.10981859594891401No Hit
AATCATAAGGATGATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGG270.10981859594891401No Hit
AAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGAC270.10981859594891401No Hit
GATGGAAAGTGCCAGACCAGAAGATGTGTCTTTCCAGGGGCGGGGAGTCT270.10981859594891401No Hit
CTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAGGAAGGCACGATC260.10575124054339868No Hit
CCTTTGACATGAGTAATGAAGGATCTTATTTCTTCGGAGACAATGCAGAG260.10575124054339868No Hit
CCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTC260.10575124054339868No Hit
ATCAGTGTACAGCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAG250.10168388513788335No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)