FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n01_3010_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n01_3010_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42500
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTA30917.272941176470588No Hit
GTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTC19424.569411764705882No Hit
GGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAA19254.529411764705882No Hit
GTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGC17074.016470588235294No Hit
AAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCT14553.4235294117647057No Hit
CCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCT13493.1741176470588237No Hit
GGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAA12172.8635294117647057No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACA11722.757647058823529No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTC10672.510588235294118No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATT10432.4541176470588235No Hit
GGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTC10202.4No Hit
CAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCC7981.8776470588235294No Hit
CAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAG7601.7882352941176471No Hit
GATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGA7561.7788235294117647No Hit
AGCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATG5891.3858823529411766No Hit
ACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTA5331.2541176470588236No Hit
AGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTC4861.143529411764706No Hit
TTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTAC4721.1105882352941177No Hit
GTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATT4611.0847058823529412No Hit
TAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCA4561.0729411764705883No Hit
ATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGAT4521.0635294117647058No Hit
CACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTC3800.8941176470588236No Hit
ATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAA3720.8752941176470588No Hit
ACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTC3420.8047058823529413No Hit
ACACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTG3390.7976470588235295No Hit
TGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTT3330.7835294117647058No Hit
CTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTC3230.76No Hit
CAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAG3220.7576470588235293No Hit
TGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATTCA3160.7435294117647059No Hit
TAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCG3100.7294117647058823No Hit
AGCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAA3040.7152941176470589No Hit
CCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGT2990.7035294117647058No Hit
TAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTC2950.6941176470588235No Hit
AATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAA2940.691764705882353No Hit
CTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTC2940.691764705882353No Hit
CATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCA2690.6329411764705882No Hit
TCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACC2670.6282352941176471No Hit
ACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCT2630.6188235294117648No Hit
AAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAA2570.6047058823529411No Hit
GAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAA2410.5670588235294117No Hit
CTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAG2390.5623529411764706No Hit
CATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTG2270.5341176470588236No Hit
AAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCAC2250.5294117647058824No Hit
GCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGG2240.5270588235294118No Hit
TCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCT2230.5247058823529412No Hit
CTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAA2060.48470588235294115No Hit
ACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACT1990.4682352941176471No Hit
GAGCGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCAT1890.4447058823529412No Hit
CAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACAC1800.4235294117647058No Hit
CTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTG1750.411764705882353No Hit
CCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAA1720.40470588235294114No Hit
GAGCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCAT1620.3811764705882353No Hit
ACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGA1610.37882352941176467No Hit
TCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAA1570.36941176470588233No Hit
GTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACAT1530.36No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAA1520.35764705882352943No Hit
GAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGA1500.35294117647058826No Hit
GCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACA1430.33647058823529413No Hit
CATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAATT1390.32705882352941174No Hit
AGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCA1320.31058823529411766No Hit
CCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCA1290.3035294117647059No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCT1260.29647058823529415No Hit
CTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCT1260.29647058823529415No Hit
AGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGA1200.2823529411764706No Hit
GCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAA1120.2635294117647059No Hit
AAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTT1120.2635294117647059No Hit
CACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTT1020.24No Hit
ATTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAAT1020.24No Hit
AAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACA960.22588235294117645No Hit
TGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCT950.2235294117647059No Hit
CAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAGATATG950.2235294117647059No Hit
AAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAACAG940.22117647058823528No Hit
ATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGT930.2188235294117647No Hit
CAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGT930.2188235294117647No Hit
CGAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGT920.2164705882352941No Hit
GATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGAA920.2164705882352941No Hit
GAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTC920.2164705882352941No Hit
AGCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATG860.20235294117647057No Hit
AAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCAT860.20235294117647057No Hit
GCAAAAGCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGG850.2No Hit
ACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATG850.2No Hit
GCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAAC820.19294117647058823No Hit
GCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCC810.19058823529411764No Hit
GCTCTCATTTGAAGTAGGAACATGCTCTACTAGGTAAACATGATGTGGAC790.18588235294117647No Hit
GTAGGAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTC790.18588235294117647No Hit
TTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAA790.18588235294117647No Hit
CACAACAGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGA780.18352941176470589No Hit
ATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCAC770.1811764705882353No Hit
GTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTCTATCT760.17882352941176471No Hit
GGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTAC750.17647058823529413No Hit
CTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTC750.17647058823529413No Hit
GTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAA690.1623529411764706No Hit
ATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGAACA670.15764705882352942No Hit
TTCTACACCACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATT670.15764705882352942No Hit
GTGTAGAATGGTCTGAAAGTCCACATCATGTTTACCTAGTAGAGCATGTT670.15764705882352942No Hit
CTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAA660.15529411764705883No Hit
GCTAATCATGCGTATTGAACTAAACACAACAGATATGTATTTCAAAAAAA650.15294117647058825No Hit
GCTCCCCGCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCAC650.15294117647058825No Hit
GCAGGCAGTAAGTGAACTTGGGAATTCTCACTCCTTTTGCATGGTCTTCT620.1458823529411765No Hit
GCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACATCATGTTTAC620.1458823529411765No Hit
ACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTCTGAAAGTCCACA610.1435294117647059No Hit
GCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTC610.1435294117647059No Hit
GAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACG610.1435294117647059No Hit
ACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAG610.1435294117647059No Hit
GGGAATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTA590.1388235294117647No Hit
AGATATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGG580.13647058823529412No Hit
GAGCGAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGA580.13647058823529412No Hit
ACCACAGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGA570.13411764705882354No Hit
CTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCTC560.13176470588235295No Hit
GTATTGAACTAAACACAACAGATATGTATTTCAAAAAAAAAAAATTCAAC550.12941176470588237No Hit
GGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAG550.12941176470588237No Hit
GAACTAAACACAACAGATATGTATTTCAAAAAAAAAAAATTCAACAAGCT540.12705882352941178No Hit
GAACATGCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACA520.12235294117647058No Hit
TTCCCTCACATAACTGTTACGTACCTTTTCCTCTGCTCCCCGCTCAAGTT520.12235294117647058No Hit
GAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGAACTAAACACAAC510.12No Hit
TTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTACCTTTTCCTCTG490.11529411764705881No Hit
ATTCTCACTCCTTTTGCATGGTCTTCTTTCCCTCACATAACTGTTACGTA480.11294117647058823No Hit
AGATAGAGCTGCTGTGGGTTTTACACATAACTAATTCCGCAGAGATGAAC450.10588235294117646No Hit
TACGTACCTTTTCCTCTGCTCCCCGCTCAAGTTCATCTCTGCGGAATTAG450.10588235294117646No Hit
GCTCTACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCA440.10352941176470587No Hit
GAGCAAAAGCAGGAAAATGCAGTCGTAGCCTGGCTAATCATGCGTATTGA440.10352941176470587No Hit
GTTATGTGTAAAACCCACAGCAGCTCTATCTGTGGTGGTGTAGAATGGTC440.10352941176470587No Hit
ACTAAACACAACAGATATGTATTTCAAAAAAAAAATTCAACAAGCTCTCA430.10117647058823528No Hit
GCTCAAGTTCATCTCTGCGGAATTAGTTATGTGTAAAACCCACAGCAGCT430.10117647058823528No Hit
TATGTATTTCAAAAAAAAAAATTCAACAAGCTCTCATTTGAAGTAGGAAC430.10117647058823528No Hit
GATGTGGACTTTCAGACCATTCTACACCACCACAGATAGAGCTGCTGTGG430.10117647058823528No Hit
ACTAGGTAAACATGATGTGGACTTTCAGACCATTCTACACCACCACAGAT430.10117647058823528No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)