FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n01_3000_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n01_3000_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276939
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG64140.2816939759914517No Hit
GAGCAAAAGCAGGACGGATAGGCAAGAGTAGGCTGGAGCAAACGCGCCAG48490.21296134854732604No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA46430.20391411451953695No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT44070.1935493221381864No Hit
GTCTTCCGACTGGGCTCTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT43690.1918804148903418No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT35700.15678944407382017No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG34470.15138745482421795No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC33250.14602938418640113No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA33250.14602938418640113No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG33130.1455023608449765No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA31950.14031996465430124No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA31740.13939767380680818No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC29760.13070178867330218No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT28070.12327954328157233No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA27740.12183022909265466No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC27280.119809972950527No Hit
GGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATT26120.11471541398342247No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT24930.1094890991809618No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT24410.10720533136812185No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT24370.1070296569209803No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC23430.10290130741315423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)