FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n01_2882_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n01_2882_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756739
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT117440.4260105871466251No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG116590.4229272339528697No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA99020.35919250970077327No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG71690.260053635835674No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT70160.2545036000869143No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG68890.2498967076680092No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA60480.21938964842155895No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA57810.20970429191882145No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC55150.20005521015953998No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG52500.19044240314371438No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA51340.18623453290282468No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG50000.18137371727972798No Hit
GTATACATTGTGGTCATAAGTTCCATTCCTGATTGACCCCATGCAGGCAT48790.17698447332155856No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT44380.16098731145748654No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA42480.15409511020085687No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA41770.15151960341548476No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT40200.14582446869290128No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTC39750.14419210523738374No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG39430.14303131344679348No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG39070.14172542268237942No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC38590.13998423499649404No Hit
GTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGCGTTTCCTGGG37290.13526851834722112No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA36950.13403517706971896No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT36870.1337449791220714No Hit
GTTCAAGAGTGCTTGATCCCATATTATCCATGTTCTCATTTGAAGCAATT35400.1284125918340474No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG34720.12594590927904312No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA34350.12460374377117311No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA34210.12409589736278986No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC33810.12264490762455205No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA33650.12206451172925692No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC33310.12083117045175477No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT32870.11923508173969316No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG31770.11524485995953915No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC31660.11484583778152374No Hit
GTATTATTATCTCCTGAGGAAGTCAGTGAAACACAGGGAACTGAAAGACT31630.11473701355115593No Hit
CTCAGTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC31510.11430171662968458No Hit
TGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACGATCAAGAATCCA31420.11397524393858105No Hit
GATACTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT31380.11383014496475727No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC31080.1127419026610789No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT31050.11263307843071106No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT31040.11259680368725512No Hit
GATCTGCACTGATACTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCT30400.1102752201060746No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA29880.10838893344636542No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG29620.10744579011651084No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG29270.10617617409555274No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT28540.10352811782326872No Hit
CCTGTATACATTGTGGTCATAAGTTCCATTCCTGATTGACCCCATGCAGG28430.10312909564525333No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA28000.10156928167664767No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA27870.10109771001172037No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT27760.10069868783370496No Hit
ATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTCGACAG27710.10051731411642524No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC27710.10051731411642524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)