FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n01_2858_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n01_2858_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038440
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG105070.5154431820411688No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT92120.4519142089048488No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA68360.33535448676438845No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG55390.27172739938384255No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT52690.2584819764133357No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG50290.2467082671062185No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG42020.20613802711877707No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC41950.20579462726398617No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG41320.20270402857086792No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA38950.1910774906300897No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA37200.18249249426031672No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT37010.18156040894016992No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA36050.17685092521732307No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT35460.17395655501265672No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA33220.16296775965934734No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC33110.16242813131610448No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC31120.1526657640156198No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT30710.15065442200898727No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA30330.1487902513686937No Hit
GTATACATTGTGGTCATAAGTTCCATTCCTGATTGACCCCATGCAGGCAT30010.14722042346107808No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA29780.14609210965247935No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT29450.14447322462275072No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG29120.1428543395930221No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC28910.14182414002864935No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT28660.14059771197582466No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC27370.13426934322324915No Hit
GTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGCGTTTCCTGGG27280.13382782912423227No Hit
GTCCTGAATTCTCCCTTCTACTTCTGAGAACTCTTTTTCAATCTGATGGA26240.12872588842448146No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG26040.12774474598222169No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC25990.12749946037165677No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA25840.12676360353996194No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG25780.12646926080728402No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT25710.1261258609524931No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC25600.12558623260925023No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA25530.1252428327544593No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA25110.1231824336257138No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT25020.12274091952669688No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC24820.12175977708443711No Hit
TGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACGATCAAGAATCCA24820.12175977708443711No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA24550.12043523478738644No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA23300.11430309452326289No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT23210.11386158042424599No Hit
CATACGGGGCATGTCCCAGATATGTTAAGCAAAGCACTCTGAAATTGGCA23070.11317478071466416No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG22090.10836718274759129No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA22060.10822001138125234No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG21770.10679735483997567No Hit
ATGTTAAGCAAAGCACTCTGAAATTGGCAACAGGAATGCGAAATGTACCA21690.10640489786307177No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA21440.10517846981024705No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTTCAAACAG21430.10512941268813407No Hit
CTCAGTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC21380.10488412707756911No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT21250.10424638449010026No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACATGTTGGGAACAA21210.10405015600164831No Hit
TTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA21030.10316712780361453No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA20980.10292184219304959No Hit
ATATAAGGTTCTTTCCATCCAAAGAATGTTTTTATGCACTTGATCGCATC20900.10252938521614569No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG20890.1024803280940327No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAATAAGCGGATCACT20750.10179352838445087No Hit
CAATAAGCGGATCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTG20470.10041992896528719No Hit
GTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCAC20450.10032181472106122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)