FastQCFastQC Report
Sun 7 Nov 2021
HF7KYBGXK_n01_2855_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7KYBGXK_n01_2855_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255453
Sequences flagged as poor quality0
Sequence length151
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGAGCGAAAGCAG9440.36953960219688164No Hit
CCCCCCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGC8230.32217276759325586No Hit
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAAT7290.2853753919507698No Hit
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGTGGGGGGAGCGAAAGC6800.26619378124351645No Hit
CCCCACGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGC6720.26306208969947503No Hit
GAGCAAAAGCAGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCG6640.2599303981554337No Hit
CCCGTGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGG6400.2505353235233096No Hit
CTAATAACCCGTGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCC6270.24544632476424236No Hit
GCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAATAACCCGGGGGG5660.2215671767409269No Hit
CCCGTGGGGGGAGCGAAAGCAGGCCTTGTTTCTACTAATAACCCGGGGGG5560.2176525623108752No Hit
CCCGTGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGA5420.2121721021088028No Hit
GAGCAAAAGCAGGGGGGAGCAAAAGCAGCCTTGTTTCTACTAATAACCCG5310.2078660262357459No Hit
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGAGCAAAAGCAG4450.17420034213730118No Hit
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGGAGCGAAAGCA4060.15893334586009952No Hit
CCCGTGGGGGGAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGG3870.15149557844300127No Hit
CCCGTGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCGTGGGGG3740.14640657968393403No Hit
GCTTTCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3710.14523219535491852No Hit
CTAATAACCCGTGGGGGGAGCAAAAGCAGCCTTGTTTCTACTAATAACCC3670.14366634958289784No Hit
CCCGTGGGGGGAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGA3620.14170904236787196No Hit
GGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCTGCTTTC3590.14053465803885648No Hit
GAGCAAAAGCAGGCCTTGTTTCTACTAATAACCCGGGGGGAGCGAAAGCA3490.13662004360880475No Hit
GCCTTGTTTCTACTAATAACCCGGGGGGAGCGAAAGCAGCCTTGTTTCTA3440.1346627363937789No Hit
GCTTTCGCTCCCCCCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAG3420.13387981350776856No Hit
CCCGTGGGGGGAGCGAAAGCAGGGGGGAGCGAAAGCAGCCTTGTTTCTAC3350.13113958340673235No Hit
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGTGGGGGGAGCAAAAGC3210.12565912320465997No Hit
GAGCAAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGGGAGCGAAAGC3210.12565912320465997No Hit
CTAATAACCCGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCGG3210.12565912320465997No Hit
CCCGTGGGGGGAGCAAAAGCAGGCCTTGTTTCTACTAATAACCCGGGGGG3190.1248762003186496No Hit
ACTAATAACCCGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCG3130.12252743166061858No Hit
CTAATAACCCGTGGGGGGAGCGAAAGCAGGCCTTGTTTCTACTAATAACC2890.11313235702849447No Hit
ACTAATAACCCGTGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACC2800.10960920404144794No Hit
CCCCCCCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGG2710.1060860510544014No Hit
GCCTTGTTTCTACTAATAACCCGTGGGGGGAGCGAAAGCAGCCTTGTTTC2640.10334582095336521No Hit
GAGCGAAAGCAGCCTTGTTTCTACTAATAACCCGGGGGGAGCGAAAGCAG2590.10138851373833935No Hit
GAGCGAAAGCAGGGGGGAGCGAAAGCAGCCTTGTTTCTACTAATAACCCG2590.10138851373833935No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)