FastQCFastQC Report
Thu 22 Jan 2015
lane2_Undetermined_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232898
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1061323.2828749932722903No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC75130.23239211382480984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG9000.083.529741
ATCGGGA11900.063.7777062
GAGGGGC13350.056.6246269
GAGCGGC14300.037.1056569
ACATGGG9950.035.9434241
CGGGAGA28300.031.7067784
GAACGGG11750.030.4371971
ACGGGAG11550.025.2666053
AACGGGA15300.023.8485032
CTCGGGG11050.022.81743435-39
AGAGGGG38500.022.4846538
TCGGGAG31200.022.4484083
TCGGGGG18500.022.30165935-39
GAGAGGG32500.021.9384757
GGGAGAG47300.021.720335
AGAGCGG29850.020.4708068
GGAGAGC27550.018.7514936
GATCCGA7700.018.0098171
GATCCGG8150.017.9109571
ATCCGGA8900.017.2191522
Produced by FastQC (version 0.11.2)