FastQCFastQC Report
Thu 22 Jan 2015
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232898
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT194930.6029574703563181TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC64420.19926394213488952TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC129750.045.0357558
GAGCACA146000.040.2179769
GATCGGA162200.037.252171
TCGGAAG164750.036.4453663
CGGAAGA165550.035.8820344
ATCGGAA168400.035.6131322
AAGAGCA170300.034.813247
GAAGAGC181000.032.7159426
GGAAGAG188900.031.5974645
ACATGGG10800.031.1554571
CACGTGA31550.022.58149730-34
TCACGTG31450.022.33678430-34
TCGGACC1800.004828703619.7465178
TCGACCG2257.8447565E-418.9566579
AACGATC32450.018.55493535-39
GCGTCGG2701.2559921E-418.4300828
CTGCCGG13350.018.1046716
CGATCTC33450.017.70003340-44
TGCCGGA13700.017.6421437
TGAAACG39800.017.59607535-39
Produced by FastQC (version 0.11.2)