FastQCFastQC Report
Thu 22 Jan 2015
lane1_Undetermined_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6459552
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1323932.0495693819014074No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199780.309278414354432No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC129480.20044733752433602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGG26200.023.0348993
CCCGCAT12450.022.1985551
CGCACGG20250.019.0309433
CGCAGGG27500.018.5086543
GCTAGGA18950.018.0384461
CCGCAGG25450.017.7152882
CCGCACG22200.017.3606992
CCGCATG41050.015.0574032
CTATTTG59300.014.59495451
GGAATGG43950.014.3877328
GCATGGA42100.014.3354884
CGCCCGG9458.367351E-1113.0805673
GAATGGG48550.012.872559
GCACGGA40900.012.8004634
TTGGTCT65350.011.0148415
GGTCTTT69200.010.8192677
GCCGCAG27200.010.6955131
CCCACGG16700.010.4497113
TATTTGG78400.010.1100852
TGGTCTT71300.010.0933036
Produced by FastQC (version 0.11.2)