FastQCFastQC Report
Thu 22 Jan 2015
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6459552
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATTGAGTATAGCCTTGGAGCATACCTCTGAAATTGGCATTCTGTA163200.2526491001233522No Hit
CTATGCGTTAGTAAAAGAATAAGTGTGAAAAAAAAAAAAATAGAATAGCG140330.21724416801660548No Hit
CTCTAATTTCATCTATCTCAGTATTTTCAAAATTTGACAATAAGAGAGAG137620.21304883063097874No Hit
CTCTTCGAAACAGCAAATGGTTAACTATGCGTTAGTAAAAGAATAAGTGT134000.2074447268169681No Hit
CTCTAAGTTGGACTTTTCATGCTCATTGTTGACTCTAATTTCATCTATCT133580.20679452692694478No Hit
CTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCAAAGCGTTT124790.19318677208574217No Hit
AGCTTGGACTTGTTCTTGAGCTTGGACTTGTTCTTGAGCTGGAGCTTGTA110780.17149796146853527No Hit
CTTTTCATGCTCATTGTTGACTCTAATTTCATCTATCTCAGTATTTTCAA108820.16846369531509306No Hit
CTTCCGAAAATGCAACGCGAGCTGCGCACATACAGCTCACTGTTCACGTC107290.16609511000143662No Hit
CTATCTCAGTATTTTCAAAATTTGACAATAAGAGAGAGTTCCTATTATTG103710.16055292998647586No Hit
CAAAGCGTTTCCGAAAACGAGCGCTTCCGAAAATGCAACGCGAGCTGCGC103680.16050648713718846No Hit
CAAAGCGTTTCCGAAAACGAGCGTTTCCGAAAATGCAACGCGAGCTGCGC100400.15542873561510148No Hit
CTCAGTATTTTCAAAATTTGACAATAAGAGAGAGTTCCTATTATTGAGTA99460.15397352633743022No Hit
CTATTACATTATCAATCCTTGTATTTCAGCTTCCGTTGAAAACGATGACT99300.15372583114123084No Hit
CTATAAATATTATTATCATCGTTTTATATGTTGATATTCATTGATCCTAT96160.1488648129158183No Hit
CTCATTGTTGACTCTAATTTCATCTATCTCAGTATTTTCAAAATTTGACA92620.14338455669990735No Hit
CGAAAATGCAACGCGAGCTGCGCACATACAGCTCACTGTTCACGTCGCAC87910.13609302936178858No Hit
CAATAAGAGAGAGTTCCTATTATTGAGTATAGCCTTGGAGCATACCTCTG85860.1329194346604842No Hit
CTAGTTAGTAGATGATAGTTGATTTTTATTCCAACAGTTACGTAAACCGT79720.12341413150633357No Hit
CTATAGGCCTTACAGTACAAAGCTACGGCTTCTTTAACATTGTCCAAATA78670.12178863178127523No Hit
CAACGCGAGCTGCGCACATACAGCTCACTGTTCACGTCGCACCTATATCT78580.1216493032334131No Hit
CTAATTTCATCTATCTCAGTATTTTCAAAATTTGACAATAAGAGAGAGTT78470.12147901278602602No Hit
CGATGATCTTGTGTGGAATCCTCTTATAAATGGAGTGAACATTTGTATGC77630.12017861300597937No Hit
CTATTTCTCATCATTTGCGTCATCTTCTAACACCGTATATGATAATATAC75570.11698953735491255No Hit
CTAAGTTGGACTTTTCATGCTCATTGTTGACTCTAATTTCATCTATCTCA75330.11661799456061349No Hit
AGAATAAGTGTGAAAAAAAAAAAAATAGAATAGCGTATGAAGAATGAACA73710.11411008069909492No Hit
AGCTTTAGTTCCCCATCTGCAGTATCATAAAGTTCTAAATGCGTCTCAGT72830.11274775711999842No Hit
CTAATAGAAAGGGAGCGTACTGAACCATGTTATCGATGATCTTGTGTGGA72440.11214400007926247No Hit
CTTCTTTAACATTGTCCAAATATTCCAATTGAATATCAGCAGCATCAACA71670.11095196694755301No Hit
AAAAGAATAAGTGTGAAAAAAAAAAAAATAGAATAGCGTATGAAGAATGA71660.11093648599779056No Hit
CTTCTAACACCGTATATGATAATATACTAGTAACGTAAACACTAGTTAGT71210.11023984325847984No Hit
ATAAGTGTGAAAAAAAAAAAAATAGAATAGCGTATGAAGAATGAACATAC69450.10751519610028683No Hit
CTTCAAAGCGTTTCCGAAAACGAGCGCTTCCGAAAATGCAACGCGAGCTG69080.10694240095907578No Hit
CTCATCATTTGCGTCATCTTCTAACACCGTATATGATAATATACTAGTAA68560.10613739157142787No Hit
CTAGTAACGTAAACACTAGTTAGTAGATGATAGTTGATTTTTATTCCAAC67600.10465122039423166No Hit
CATCTATCTCAGTATTTTCAAAATTTGACAATAAGAGAGAGTTCCTATTA66870.10352111106157207No Hit
CTTGTATTTCAGCTTCCGTTGAAAACGATGACTATTTCTCATCATTTGCG66100.10232907792986264No Hit
ATCTTCTAACACCGTATATGATAATATACTAGTAACGTAAACACTAGTTA66050.10225167318105033No Hit
CTCTTATAAATGGAGTGAACATTTGTATGCCCCTTGTGTGCGTGTGGCTA65300.10109060194886581No Hit
CTTCAAAGCGTTTCCGAAAACGAGCGTTTCCGAAAATGCAACGCGAGCTG64880.10044040205884246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCCC17050.070.168868
AGTTCCC18400.064.631267
TTCCCCA28250.043.1104779
GTGTCAT61200.040.454594145
TAGTTCC30150.039.68096
TGGCCAC20200.034.0464829
CTGGGAC22250.032.8414659
AACTGGG20050.032.514677
CTTGGAC54550.031.5409583
CTATACT62000.029.0758081
ATGGCCA24950.027.5646868
GGACTTG62400.026.6351876
TGTGCGG6800.026.16225145
GCTTGGA73800.025.9392282
GGCAGTT16700.025.744492145
TTAGTTC49850.025.166995
GAACTGG27600.023.8798246
ACTGGGA32400.023.4376058
TCTAGAG77450.022.9394289
TATGGCC31850.022.0429137
Produced by FastQC (version 0.11.2)