FastQCFastQC Report
Thu 22 Jan 2015
GS5662_AGTTCC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGS5662_AGTTCC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4403434
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC20950.0107.972549
GATCGGG25750.0106.2361451
CGGGAGA30150.094.083544
GAGCGGC18100.090.12539
AGAGCGG21750.081.334238
ATCGGGA34300.080.593412
TCGGGAG26000.077.57213
GGGAGAG43450.073.127195
GATCGGA32500.063.18461
GAGAGCG23850.062.6212357
GAACGGG21250.058.049461
ACGGGAG15800.056.9375343
GAGAGGG40700.053.084127
GGAGAGC31100.048.0224886
AAGGGGC21050.047.185799
AACGGGA26900.046.931632
CGGAAGA50050.044.789744
GAGGGTC14500.044.500499
AGAGGGG59400.043.9398738
GATCCGG10250.043.8910521
Produced by FastQC (version 0.11.2)