FastQCFastQC Report
Thu 22 Jan 2015
GS5662_AGTTCC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGS5662_AGTTCC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4403434
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT942032.1393076403552316TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA128650.0123.308221
GAGCACA134500.0118.255339
AGAGCAC143150.0111.1590048
TCGGAAG143950.0110.297943
CGGAAGA144150.0110.044274
ATCGGAA147250.0107.924592
GAAGAGC163550.097.781446
GGAAGAG166750.095.129735
AAGAGCA183950.087.016337
GCACACG120850.026.40643710-14
GCCGTCT120150.026.16176250-54
CACACGT123250.025.96281610-14
ACACGTC123650.025.96090910-14
GTCACAG121150.025.92411625-29
TGCCGTC120600.025.82369850-54
AGTTCCG122000.025.58829330-34
ACGTCTG125700.025.51543615-19
TATGCCG124900.025.51506845-49
CAGTCAC125300.025.40109325-29
CCGTATC120600.025.25865435-39
Produced by FastQC (version 0.11.2)