FastQCFastQC Report
Thu 22 Jan 2015
GS5082_AGTCAA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGS5082_AGTCAA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524447
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTTT161300.016.8262881
AGCTTCC56100.016.5576321
AGCTTCG37200.015.6062351
AGCTTGG47350.015.1728221
AGCTTAT80950.014.7917051
AGCTTGC41650.013.2418821
AGCTTTG82400.012.9461691
AGCTTGA77800.012.3124771
AGCTTCT83850.012.0299231
AGCTTGT64150.011.7648871
CTATTAA105500.011.5560111
AGCTTCA85950.011.4827031
GCTTATG48100.011.162732
CTATAAG26300.011.037111
AGCTTTA114000.010.8216921
AGCTTAC40650.010.3542481
CTATGAG23300.010.2780121
CTATAAT73850.010.023071
AGCTTTC77950.09.5889761
CTATACG14701.3278623E-99.3796661
Produced by FastQC (version 0.11.2)